ArticleCell cycle (Georgetown, Tex.)2014
iRFP is a sensitive marker for cell number and tumor growth in high-throughput systems.
Article in Cell cycle (Georgetown, Tex.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- TRACERx analysis identifies a role for FAT1 in regulating chromosomal instability and whole-genome doubling via Hippo signalling.Nature cell biology · 2025Article
- Loss of attachment promotes proline accumulation and excretion in cancer cells.Science advances · 2023Article
- A noninvasive iRFP713 p53 reporter reveals dynamic p53 activity in response to irradiation and liver regeneration in vivo.Science signaling · 2022Article
- Image-guided surgery with a new tumour-targeting probe improves the identification of positive margins.EBioMedicine · 2022Article
- A real-time and high-throughput neutralization test based on SARS-CoV-2 pseudovirus containing monomeric infrared fluorescent protein as reporter.Emerging microbes & infections · 2021Article
- Induced pluripotent stem cells can improve thrombolytic effect of low-dose rt-PA after acute carotid thrombosis in rat.Stem cell research & therapy · 2021Article
- Breast cancer dependence on MCL-1 is due to its canonical anti-apoptotic function.Cell death and differentiation · 2021Article
- iRFP (near-infrared fluorescent protein) imaging of subcutaneous and deep tissue tumours in mice highlights differences between imaging platforms.Cancer cell international · 2021Article
- Quantitative in vivo bioluminescence imaging of orthotopic patient-derived glioblastoma xenografts.Scientific reports · 2020Article
- Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.Cell reports · 2020Article
- Development and Optimization of a Miniaturized Western Blot-Based Screening Platform to Identify Regulators of Post-Translational Modifications.High-throughput · 2019Article
- Persistent double strand break accumulation does not precede cell death in an Olaparib-sensitive BRCA-deficient colorectal cancer cell model.Genetics and molecular biology · 2019Article
- Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.Trends in biotechnology · 2018Review
- A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3.Cell metabolism · 2018Article
- Gene therapy for human glioblastoma using neurotropic JC virus-like particles as a gene delivery vector.Scientific reports · 2018Article
- p53-mediated adaptation to serine starvation is retained by a common tumour-derived mutant.Cancer & metabolism · 2018Article
- Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo.Scientific reports · 2017Article
- AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging.Bioscience reports · 2017Article
- In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
GFP and luciferase are used extensively as markers both in vitro and in vivo although both have limitations. The utility of GFP fluorescence is restricted by high background signal and poor tissue penetrance. Luciferase throughput is limited in vitro by the requirement for cell lysis, while in vivo, luciferase readout is complicated by the need for substrate injection and the dependence on endogenous ATP. Here we show that near-infrared fluorescent protein in combination with widely available near-infrared scanners overcomes these obstacles and allows for the accurate determination of cell number in vitro and tumor growth in vivo in a high-throughput manner and at negligible per-well costs. This system represents a significant advance in tracking cell proliferation in tissue culture as well as in animals, with widespread applications in cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.