ArticleJournal of neuroscience methods2014
Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope.
Article in Journal of neuroscience methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 58 citations in OpenAlex.
- Deep learning-assisted mapping of dendritic spines using sequential 2D two-photon calcium imaging.iScience · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A real-time all-optical interface for dynamic perturbation of neural activity during behavior.Cell reports methods · 2025Article
- Acousto-optic holography for pseudo-two-dimensional dynamic light patterning.APL photonics · 2024Article
- Multi-neuronal recording in unrestrained animals with all acousto-optic random-access line-scanning two-photon microscopy.Frontiers in neuroscience · 2023Article
- Dendritic Morphology of an Inhibitory Retinal Interneuron Enables Simultaneous Local and Global Synaptic Integration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Fast optical recording of neuronal activity by three-dimensional custom-access serial holography.Nature methods · 2022Article
- Cerebellar granule cell axons support high-dimensional representations.Nature neuroscience · 2021Article
- Multidimensional population activity in an electrically coupled inhibitory circuit in the cerebellar cortex.Neuron · 2021Article
- Real-time 3D movement correction for two-photon imaging in behaving animals.Nature methods · 2020Article
- The temporal structure of the inner retina at a single glance.Scientific reports · 2020Article
- Neuronal Activity at Synapse Resolution: Reporters and Effectors for Synaptic Neuroscience.Frontiers in molecular neuroscience · 2020Review
- Line excitation array detection fluorescence microscopy at 0.8 million frames per second.Nature communications · 2018Article
- A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity.Annual review of neuroscience · 2018Review
- NeuroMatic: An Integrated Open-Source Software Toolkit for Acquisition, Analysis and Simulation of Electrophysiological Data.Frontiers in neuroinformatics · 2018Article
- Studying Axon-Astrocyte Functional Interactions by 3D Two-Photon CaFrontiers in cellular neuroscience · 2018Article
- Video rate volumetric CaNature methods · 2017Article
- Analysis of the dynamics of temporal relationships of neural activities using optical imaging data.Journal of computational neuroscience · 2017Article
- Simultaneous high-speed imaging and optogenetic inhibition in the intact mouse brain.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundTwo-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded indicators have not been investigated. NEW
methodHere, we have used a compact acousto-optic lens (AOL) two-photon microscope to make high speed [Ca(2+)] measurements from spines and dendrites distributed in 3D with different excitation wavelengths (800-920 nm).
resultsWe show simultaneous monitoring of activity from many synaptic inputs distributed over the 3D arborisation of a neuronal dendrite using both synthetic as well as genetically encoded indicators. We confirm the utility of AOL-based imaging for fast in vivo recordings by measuring, simultaneously, visually evoked responses in 100 neurons distributed over a 150 μm focal depth range. Moreover, we explore ways to improve the measurement of timing of neuronal activation by choosing specific regions within the cell soma. COMPARISON WITH EXISTING
methodsThese results establish that AOL-based 3D random access two-photon microscopy has a wider range of neuroscience applications than previously shown.
conclusionsOur findings show that the compact AOL microscope design has the speed, spatial resolution, sensitivity and wavelength flexibility to measure 3D patterns of synaptic and neuronal activity on individual trials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.