ArticleStem cells and development2014
The miR-7 identified from collagen biomaterial-based three-dimensional cultured cells regulates neural stem cell differentiation.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Therapeutic Potential of Mesenchymal Stem Cells in Multiple Sclerosis: An Updated Review.Molecular neurobiology · 2026Review
- Mining of potentially stem cell-related miRNAs in planarians.Molecular biology reports · 2024Review
- Use of microRNAs as Diagnostic, Prognostic, and Therapeutic Tools for Glioblastoma.International journal of molecular sciences · 2024Review
- miR-153-3p suppresses the differentiation and proliferation of neural stem cells via targeting GPR55.Aging · 2023Article
- Article
- The Mechanosensing and Global DNA Methylation of Human Osteoblasts on MEW Fibers.Nanomaterials (Basel, Switzerland) · 2021Article
- The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen sponge.Stem cell research & therapy · 2021Article
- Surface Topography of Titanium Affects Their Osteogenic Potential through DNA Methylation.International journal of molecular sciences · 2021Article
- The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells.Chemical reviews · 2020Review
- MiR-485-3p modulates neural stem cell differentiation and proliferation via regulating TRIP6 expression.Journal of cellular and molecular medicine · 2020Article
- Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.International journal of oncology · 2018Article
- LncRNA OIP5-AS1 is overexpressed in undifferentiated oral tumors and integrated analysis identifies as a downstream effector of stemness-associated transcription factors.Scientific reports · 2018Article
- Target RNAs Strike Back on MicroRNAs.Frontiers in genetics · 2018Review
- Long Noncoding RNA Moderates MicroRNA Activity to Maintain Self-Renewal in Embryonic Stem Cells.Stem cell reports · 2017Article
- MicroRNA-802 plays a tumour suppressive role in tongue squamous cell carcinoma through directly targeting MAP2K4.Cell proliferation · 2017Article
- Microfluidic engineering of neural stem cell niches for fate determination.Biomicrofluidics · 2017Article
- Systematic Analysis of mRNA and miRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight.Frontiers in cellular neuroscience · 2017Article
- The Effects of Different Factors on the Behavior of Neural Stem Cells.Stem cells international · 2017Review
- Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression.American journal of translational research · 2017Article
- Divergent modulation of normal and neoplastic stem cells by thrombospondin-1 and CD47 signaling.The international journal of biochemistry & cell biology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increasing evidence suggests that three-dimensional (3D) cultures provide more appropriate microenvironments to control stem cell response compared with traditional two-dimensional (2D) cultures. However, the molecular mechanism involved in 3D cultured stem cells is not well known. Several microRNAs whose target genes involved in the regulation of self-renewal and differentiation of stem cells were found to be downregulated in 3D cultured PA-1 cells. Among them, miR-7 was predicted to target Kruppel-like factor 4 (Klf4), a key gene for self-renewal of neural stem cells (NSCs). We showed that the differentiation of NSCs was inhibited in 3D collagen scaffolds compared with 2D cultured cells. The quantitative real-time PCR (qPCR) analysis indicated that the expression of miR-7 and Klf4 changed significantly in 2D cultures, whereas the expression stability of miR-7 and Klf4 was detected in 3D cultures. Using luciferase assay and western blot, Klf4 was identified as a target of miR-7 indicating that miR-7 plays a critical role in maintaining the self-renewal capacity through a Klf4-dependent mechanism in 3D cultured cells. Thus, the collagen scaffold-based 3D cell cultures may provide a platform to reveal the regulatory mechanism of cell regulators, which are difficult to find in traditional 2D cell cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.