ArticleAdvances in urology2013
Assessment of chromatin maturity in human spermatozoa: useful aniline blue assay for routine diagnosis of male infertility.
Article in Advances in urology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- Large-scale transcriptomic analyses reveal downstream target genes of ZFY1 and ZFY2 transcription factors in male germ cells.Cell death and differentiation · 2026Article
- Flow Cytometric Assessment of Sperm DNA Fragmentation by TUNEL and Acridine Orange: Methodological and Clinical Insights.Journal of clinical medicine · 2026Article
- The seminal plasma sTLR4/IL-1β ratio as an independent factor associated with sperm chromatin condensation defects.Frontiers in physiology · 2026Article
- Effect of Sperm DNA Fragmentation and Chromatin Decondensation on PLCζ Efficacy in Infertile Patients.Current issues in molecular biology · 2025Article
- Impact of Type 1 Diabetes on Testicular Microtubule Dynamics, Sperm Physiology, and Male Reproductive Health in Rat.International journal of molecular sciences · 2025Article
- Methylation Status of cAMP-responsive Element Modulator (CREM) Gene in Infertile Men and Its Association with Sperm Parameters.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Endometrial scratching in unexplained repeated implantation failure causes two competing forces, angiogenesis and anti-angiogenesis: An RCT study.International journal of reproductive biomedicine · 2024Article
- Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.International journal of molecular sciences · 2023Article
- Article
- Aniline blue test for sperm protamination-a valuable add-on to the bull breeding soundness evaluation.Tropical animal health and production · 2023Article
- The effect of astaxanthin on human sperm parameters after cryopreservation.Canadian Urological Association journal = Journal de l'Association des urologues du Canada · 2022Article
- Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men.Life (Basel, Switzerland) · 2021Article
- Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function.Clinical epigenetics · 2021Article
- Discordance between human sperm quality and telomere length following differential gradient separation/swim-up.Journal of assisted reproduction and genetics · 2020Article
- Relationship between Sperm Parameters with Sperm Function Tests in Infertile Men with at Least One Failed Cycle after Intracytoplasmic Sperm Injection Cycle.International journal of fertility & sterility · 2020Article
- DNA fragmentation in concert with the simultaneous assessment of cell viability in a subfertile population: establishing thresholds of normality both before and after density gradient centrifugation.Journal of assisted reproduction and genetics · 2019Article
- Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa.Scientific reports · 2019Article
- Abnormal Human Sperm Parameters Contribute to Sperm DNA Fragmentation in Men with Varicocele.The world journal of men's health · 2018Article
- Effects of administration of co-trimoxazole and folic acid on sperm quality and histological changes of testes in male rats.International journal of reproductive biomedicine · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation. This nuclear compaction would be useful as a predictor of sperm fertilization capacity and pregnancy outcome. We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men. Secondly, we analyzed whether the sperm gradient density centrifugation is effective to select mature spermatozoa. Fifty-one ejaculates were investigated by semen analysis and stained for chromatin condensation with AB to distinguish between unstained mature sperm and stained immature sperm. AB was applied also on 12 ejaculates which proceeded by density gradient centrifugation to compare the rates of immature sperm before and after selection. Neat semen were divided into two groups: G1 (n = 31): immature sperm <20% and G2 (n = 20): immature sperm ≥20%. No significant differences were detected in sperm concentration, motility, and normal morphology between G1 and G2. However, the rates of some morphology abnormalities were higher in G2: head abnormalities (P = 0.01) and microcephalic sperm (P = 0.02). We founded significant correlation between sperm immaturity and acrosome abnormalities (r = 0.292; P = 0.03). Sperm selection has significantly reduced the rates of immature sperm. A better understanding of chromatin structure and its impact on the sperm potential is needed to explore male infertility.
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Registered trials
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