ArticleMycobiology2013
Generation and Evaluation of High β-Glucan Producing Mutant Strains of Sparassis crispa.
Article in Mycobiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Beta-Glucans in Biotechnology: A Holistic Review with a Special Focus on Yeast.Bioengineering (Basel, Switzerland) · 2025Review
- Aeronautical Mutagenesis and Whole-genome Resequencing Reveal the Genetic Basis of Color Change inMycobiology · 2025Article
- Comparative transcriptomics reveals unique pine wood decay strategies in the Sparassis latifolia.Scientific reports · 2022Article
- Excessive Oxalic Acid Secreted byCells · 2022Article
- De novo transcriptome assembly and comprehensive assessment provide insight into fruiting body formation of Sparassis latifolia.Scientific reports · 2022Article
- Edible and medicinal fungi breeding techniques, a review: Current status and future prospects.Current research in food science · 2022Review
- Alginate-Derived Elicitors Enhance β-Glucan Content and Antioxidant Activities in Culinary and Medicinal Mushroom,Journal of fungi (Basel, Switzerland) · 2020Article
- Isolation and Characterization of Monokaryotic Strains of Lentinula edodes Showing Higher Fruiting Rate and Better Fruiting Body Production.Mycobiology · 2015Article
- Cloning and Molecular Characterization of β-1,3-Glucan Synthase from Sparassis crispa.Mycobiology · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high β-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and β-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in β-glucan productivity by producing 0.254 and 0.236 mg soluble β-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble β-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
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Registered trials
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