Evidence map›Paper›PMID 24198663›Full record

ArticleJournal of ginseng research2013

Isolation and characterization of LHT-type plant amino acid transporter gene from Panax ginseng Meyer.

Ru Zhang, Jie Zhu, Hong-Zhe Cao, Xiao-Lei Xie, Jing-Jia Huang, Xiang-Hui Chen, Zhi-Yong Luo

Abstract read
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Article in Journal of ginseng research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

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16citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Genome-Wide Analysis of theInternational journal of molecular sciences · 2022
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  5. Identification, Phylogenetic and Expression Analyses of theInternational journal of molecular sciences · 2022
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  11. Evidence-based complementary and alternative medicine : eCAM · 2019
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  14. Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2015
    Article
  15. Article
  16. Article
4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Ru ZhangMolecular Biology Research Center, School of Life Sciences, Central South University, Changsha 410078, China ; College of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China.
Jie Zhu
Hong-Zhe Cao
Xiao-Lei Xie
Jing-Jia Huang
Xiang-Hui Chen
Zhi-Yong Luo

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A lysine histidine transporter (LHT) cDNA was isolated and characterized from the roots of Panax ginseng, designated PgLHT. The cDNA is 1,865 bp with an open reading frame that codes for a protein with 449 amino acids and a calculated molecular mass of 50.6 kDa with a predicted isoelectric point of 8.87. Hydropathy analysis shows that PgLHT is an integral membrane protein with 9 putative membrane-spanning domains. Multiple sequence alignments show that PgLHT shares a high homology with other plant LHTs. The expression profile of the gene was investigated by real-time quantitative polymerase chain reaction during various chemical treatments. PgLHT was up-regulated in the presence of abscisic acid, salicylic acid, methyl jasmonate, NaCl, and amino acids. To further explore the function of PgLHT gene, full-length cDNA of PgLHT was introduced into P. ginseng by Agrobacterium rhizogenes A4. The overexpression of PgLHT in the hairy roots led to an obviously increase of biomass compared to the controls, and after addition of the amino acids, the overexpressed-PgLHT hairy roots grew more rapidly than untreated controls during early stage of the culture cycle. The results suggested that the PgLHT isolated from ginseng might have role in the environmental stresses and growth response.

Indexed as

Abiotic stressesLHT genePanax ginsengReal-time quantitative polymerase chain reaction

Identifiers

PMID24198663
PMCPMC3818964

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.