ArticleJournal of virology2014
Neisseria gonorrhoeae filamentous phage NgoΦ6 is capable of infecting a variety of Gram-negative bacteria.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
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- Current alternative therapies for treating drug-resistantFuture microbiology · 2024Review
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- Sculpting the BacterialmBio · 2022Article
- Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages InfectingAntibiotics (Basel, Switzerland) · 2022Article
- A New Vaccination Method Based on Phage NgoΦ6 and Its Phagemid Derivatives.Frontiers in microbiology · 2022Article
- Potential for Phages in the Treatment of Bacterial Sexually Transmitted Infections.Antibiotics (Basel, Switzerland) · 2021Review
- First Report of Filamentous Phages Isolated from Tunisian Orchards to ControlMicroorganisms · 2020Article
- Identification of integrative and conjugative elements in pathogenic and commensalMicrobial genomics · 2020Article
- Meningococcal Disease-Associated Prophage-Like Elements Are Present in Neisseria gonorrhoeae and Some Commensal Neisseria Species.Genome biology and evolution · 2020Article
- Association of host proteins with the broad host range filamentous phage NgoΦ6 of Neisseria gonorrhoeae.PloS one · 2020Article
- Filamentous phages: masters of a microbial sharing economy.EMBO reports · 2019Review
- Application of filamentous phages in environment: A tectonic shift in the science and practice of ecorestoration.Ecology and evolution · 2019Article
- Phage proteins are expressed on the surface of Neisseria gonorrhoeae and are potential vaccine candidates.PloS one · 2018Article
- Oral Immunization of Rabbits with S. enterica Typhimurium Expressing Neisseria gonorrhoeae Filamentous Phage Φ6 Induces Bactericidal Antibodies Against N. gonorrhoeae.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
We constructed a phagemid consisting of the whole genome of the Neisseria gonorrhoeae bacteriophage NgoΦ6 cloned into a pBluescript plasmid derivative lacking the f1 origin of replication (named pBS::Φ6). Escherichia coli cells harboring pBS::Φ6 were able to produce a biologically active phagemid, NgoΦ6fm, capable of infecting, integrating its DNA into the chromosome of, and producing progeny phagemids in, a variety of taxonomically distant Gram-negative bacteria, including E. coli, Haemophilus influenzae, Neisseria sicca, Pseudomonas sp., and Paracoccus methylutens. A derivative of pBS::Φ6 lacking the phage orf7 gene, a positional homolog of filamentous phage proteins that mediate the interaction between the phage and the bacterial pilus, was capable of producing phagemid particles that were able to infect E. coli, Haemophilus influenzae, N. sicca, Pseudomonas sp., and Paracoccus methylutens, indicating that NgoΦ6 infects cells of these species using a mechanism that does not involve the Orf7 gene product and that NgoΦ6 initiates infection through a novel process in these species. We further demonstrate that the establishment of the lysogenic state does not require an active phage integrase. Since phagemid particles were capable of infecting diverse hosts, this indicates that NgoΦ6 is the first broad-host-range filamentous bacteriophage described.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.