ArticleNucleic acids research2014
Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Structure of the Nmd4-Upf1 complex supports conservation of the nonsense-mediated mRNA decay pathway between yeast and humans.PLoS biology · 2024Article
- Tracking endogenous proteins based on RNA editing-mediated genetic code expansion.Nature chemical biology · 2024Article
- Characterization of the mIF4G Domains in the RNA Surveillance Protein Upf2p.Current issues in molecular biology · 2023Article
- UPF1-From mRNA Degradation to Human Disorders.Cells · 2023Review
- Global SLAM-seq for accurate mRNA decay determination and identification of NMD targets.RNA (New York, N.Y.) · 2022Article
- Molecular Determinants and Specificity of mRNA with Alternatively-Spliced UPF1 Isoforms, Influenced by an Insertion in the 'Regulatory Loop'.International journal of molecular sciences · 2021Article
- A role for AKT1 in nonsense-mediated mRNA decay.Nucleic acids research · 2021Article
- Catalytic Enantiodivergent Michael Addition by Subtle Adjustment of Achiral Amino Moiety of Dipeptide Phosphines.iScience · 2020Article
- Dbp5/DDX19 between Translational Readthrough and Nonsense Mediated Decay.International journal of molecular sciences · 2020Review
- Nonsense-mediated mRNA decay involves two distinct Upf1-bound complexes.The EMBO journal · 2018Article
- The evolution and diversity of the nonsense-mediated mRNA decay pathway.F1000Research · 2018Review
- Conservation of Nonsense-Mediated mRNA Decay Complex Components Throughout Eukaryotic Evolution.Scientific reports · 2017Article
- Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay.Nature communications · 2016Article
- The RNA helicase DHX34 functions as a scaffold for SMG1-mediated UPF1 phosphorylation.Nature communications · 2016Article
- In Silico Analysis of the Structural and Biochemical Features of the NMD Factor UPF1 in Ustilago maydis.PloS one · 2016Article
- Mechanism, factors, and physiological role of nonsense-mediated mRNA decay.Cellular and molecular life sciences : CMLS · 2015Review
- Review
- Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.Annual review of genetics · 2015Review
- Phosphoproteomics technologies and applications in plant biology research.Frontiers in plant science · 2015Review
- Biological and clinical significance of epigenetic silencing of MARVELD1 gene in lung cancer.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
One third of inherited genetic diseases are caused by mRNAs harboring premature termination codons as a result of nonsense mutations. These aberrant mRNAs are degraded by the Nonsense-Mediated mRNA Decay (NMD) pathway. A central component of the NMD pathway is Upf1, an RNA-dependent ATPase and helicase. Upf1 is a known phosphorylated protein, but only portions of this large protein have been examined for phosphorylation sites and the functional relevance of its phosphorylation has not been elucidated in Saccharomyces cerevisiae. Using tandem mass spectrometry analyses, we report the identification of 11 putative phosphorylated sites in S. cerevisiae Upf1. Five of these phosphorylated residues are located within the ATPase and helicase domains and are conserved in higher eukaryotes, suggesting a biological significance for their phosphorylation. Indeed, functional analysis demonstrated that a small carboxy-terminal motif harboring at least three phosphorylated amino acids is important for three Upf1 functions: ATPase activity, NMD activity and the ability to promote translation termination efficiency. We provide evidence that two tyrosines within this phospho-motif (Y-738 and Y-742) act redundantly to promote ATP hydrolysis, NMD efficiency and translation termination fidelity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.