ArticleWorld journal of microbiology & biotechnology2014
Co-expression of RCH10 and AGLU1 confers rice resistance to fungal sheath blight Rhizoctonia solani and blast Magnorpathe oryzae and reveals impact on seed germination.
Article in World journal of microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Involvement of Phytohormone Responses of Rubber Leaves after Pestalotiopsis microspora Infection Revealed with Transcriptome Analysis.The plant pathology journal · 2026Article
- Overexpression ofJournal of fungi (Basel, Switzerland) · 2025Article
- Proteomics analysis revealed the activation and suppression of different host defense components challenged with mango leaf spot pathogen Alternaria alternata.BMC plant biology · 2025Article
- Advances in breeding, biotechnology, and nanotechnological approaches to combat sheath blight disease in rice.Molecular biology reports · 2024Review
- Ectopic Expression ofJournal of fungi (Basel, Switzerland) · 2023Article
- Physiological and metabolic analyses provide insight into soybean seed resistance toFrontiers in plant science · 2022Article
- Loose Plant Architecture 1-Interacting Kinesin-like Protein KLP Promotes Rice Resistance to Sheath Blight Disease.Rice (New York, N.Y.) · 2021Article
- Protein Phosphatase 2A Catalytic Subunit PP2A-1 Enhances Rice Resistance to Sheath Blight Disease.Frontiers in genome editing · 2021Article
- Understanding sheath blight resistance in rice: the road behind and the road ahead.Plant biotechnology journal · 2020Review
- Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease.Rice (New York, N.Y.) · 2020Article
- Article
- Genome-Wide Association Analysis of the Genetic Basis for Sheath Blight Resistance in Rice.Rice (New York, N.Y.) · 2019Article
- Review
- Inhibition of OsSWEET11 function in mesophyll cells improves resistance of rice to sheath blight disease.Molecular plant pathology · 2018Article
- Prospects of Understanding the Molecular Biology of Disease Resistance in Rice.International journal of molecular sciences · 2018Review
- Co-transformation mediated stacking of blast resistance genes Pi54 and Pi54rh in rice provides broad spectrum resistance against Magnaporthe oryzae.Plant cell reports · 2017Article
- Dual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice.Scientific reports · 2017Article
- Co-transformation of canola by chimeric chitinase and tlp genes towards improving resistance to Sclerotinia sclerotiorum.World journal of microbiology & biotechnology · 2016Article
- Article
- Functional analysis of OsPGIP1 in rice sheath blight resistance.Plant molecular biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice sheath blight and blast caused by Rhizoctonia solani Kühn and Magnorpathe oryzae respectively, are the two most destructive fungal diseases in rice. With no genetic natural traits conferring resistance to sheath blight, transgenic manipulation provides an obvious approach. In this study, the rice basic chitinase gene (RCH10) and the alfalfa β-1,3-glucanase gene (AGLU1) were tandemly inserted into transformation vector pBI101 under the control of 35S promoter with its enhancer sequence to generate a double-defense gene expression cassette pZ100. The pZ100 cassette was transformed into rice (cv. Taipei 309) by Agrobacterium-mediated transformation. More than 160 independent transformants were obtained and confirmed by PCR. Northern analysis of inheritable progenies revealed similar levels of both RCH10 and AGLU1 transcripts in the same individuals. Disease resistance to both sheath blight and blast was challenged in open field inoculation. Immunogold detection revealed that RCH10 and AGLU1 proteins were initially located mainly in the chloroplasts and were delivered to the vacuole and cell wall upon infection, suggesting that these subcellular compartments act as the gathering and execution site for these anti-fungal proteins. We also observed that transgenic seeds display lower germination rate and seedling vigor, indicating that defense enhancement might be achieved at the expense of development.
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