ArticleJournal of industrial microbiology & biotechnology2014
MbtH homology codes to identify gifted microbes for genome mining.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Variovoracins: new glycolipopeptide biosurfactants identified by surfactant screening and genome mining.RSC advances · 2026Article
- Micrococcus spp. as a promising source for drug discovery: A review.Journal of industrial microbiology & biotechnology · 2023Review
- Genome mining for drug discovery: cyclic lipopeptides related to daptomycin.Journal of industrial microbiology & biotechnology · 2021Article
- Natural product drug discovery in the genomic era: realities, conjectures, misconceptions, and opportunities.Journal of industrial microbiology & biotechnology · 2019Article
- Molecular beacons to identify gifted microbes for genome mining.The Journal of antibiotics · 2017Article
- Gifted microbes for genome mining and natural product discovery.Journal of industrial microbiology & biotechnology · 2017Review
- Non-ribosomal peptide synthetases: Identifying the cryptic gene clusters and decoding the natural product.Journal of biosciences · 2017Review
- Structural insight into the necessary conformational changes of modular nonribosomal peptide synthetases.Current opinion in chemical biology · 2016Review
- An Activator of an Adenylation Domain Revealed by Activity but Not Sequence Homology.Chembiochem : a European journal of chemical biology · 2016Article
- Natural product discovery: past, present, and future.Journal of industrial microbiology & biotechnology · 2016Review
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis.Applied and environmental microbiology · 2016Article
- Microbial genome mining for accelerated natural products discovery: is a renaissance in the making?Journal of industrial microbiology & biotechnology · 2014Review
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in DNA sequencing technologies have made it possible to sequence large numbers of microbial genomes rapidly and inexpensively. In recent years, genome sequencing initiatives have demonstrated that actinomycetes with large genomes generally have the genetic potential to produce many secondary metabolites, most of which remain cryptic. Since the numbers of new and novel pathways vary considerably among actinomycetes, and the correct assembly of secondary metabolite pathways containing type I polyketide synthase or nonribosomal peptide synthetase (NRPS) genes is costly and time consuming, it would be advantageous to have simple genetic predictors for the number and potential novelty of secondary metabolite pathways in targeted microorganisms. For secondary metabolite pathways that utilize NRPS mechanisms, the small chaperone-like proteins related to MbtH encoded by Mycobacterium tuberculosis offer unique probes or beacons to identify gifted microbes encoding large numbers of diverse NRPS pathways because of their unique function(s) and small size. The small size of the mbtH-homolog genes makes surveying large numbers of genomes straight-forward with less than ten-fold sequencing coverage. Multiple MbtH orthologs and paralogs have been coupled to generate a 24-mer multiprobe to assign numerical codes to individual MbtH homologs by BLASTp analysis. This multiprobe can be used to identify gifted microbes encoding new and novel secondary metabolites for further focused exploration by extensive DNA sequencing, pathway assembly and annotation, and expression studies in homologous or heterologous hosts.
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