ArticleVirchows Archiv : an international journal of pathology2013
High stability of microRNAs in tissue samples of compromised quality.
Article in Virchows Archiv : an international journal of pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Differentially Expressed miRNAs in Ulcerative Colitis and Crohn's Disease.Frontiers in immunology · 2022Pooled it
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- miRNA expression profile as a potential tool for discrimination between bacterial and interstitial cystitis.Frontiers in immunology · 2026Article
- Pharmaco-Epigenetics and Epigenetic Drugs in Type 2 Diabetes: Can Epigenetics Predict Drug Efficiency?Biomedicines · 2025Review
- Epigenetic analyses in forensic medicine: future and challenges.International journal of legal medicine · 2024Review
- Development of a Novel Endometrial Signature Based on Endometrial microRNA for Determining the Optimal Timing for Embryo Transfer.Biomedicines · 2024Article
- Adipose and Plasma microRNAs miR-221 and 222 Associate with Obesity, Insulin Resistance, and New Onset Diabetes after Peritoneal Dialysis.Nutrients · 2022Article
- Development of a miRNA-based classifier for detection of colorectal cancer molecular subtypes.Molecular oncology · 2022Article
- MicroRNA expression profiling with a droplet digital PCR assay enables molecular diagnosis and prognosis of cancers of unknown primary.Molecular oncology · 2021Article
- Comparing the Predictivity of Human Placental Gene, microRNA, and CpG Methylation Signatures in Relation to Perinatal Outcomes.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Is there a correlation between miR-301a expression and neoadjuvant chemotherapy efficacy in breast cancer tissue?Biochemistry and biophysics reports · 2021Article
- Precision Medicine and Public Health: New Challenges for Effective and Sustainable Health.Journal of personalized medicine · 2021Review
- Review
- miR-122 and miR-197 expressions in hepatic carcinoma patients before and after chemotherapy and their effect on patient prognosis.American journal of translational research · 2021Article
- MicroRNAs: An Update of Applications in Forensic Science.Diagnostics (Basel, Switzerland) · 2020Review
- miRNA profiles of canine cutaneous mast cell tumours with early nodal metastasis and evaluation as potential biomarkers.Scientific reports · 2020Article
- Reliability of miRNA Analysis from Fixed and Paraffin-Embedded Tissues.International journal of molecular sciences · 2019Article
- Repression of miR-135b-5p promotes metastasis of early-stage breast cancer by regulating downstream target SDCBP.Laboratory investigation; a journal of technical methods and pathology · 2019Observational
- Small Nucleolar RNA Score: An Assay to Detect Formalin-Overfixed Tissue.Biopreservation and biobanking · 2018Article
- Method Optimization for Extracting High-Quality RNA From the Human Pancreas Tissue.Translational oncology · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Degradation of tissue samples limits performing RNA-based molecular studies, but little is known about the potential usefulness of samples of compromised quality for studies focused on miRNAs. In this work we analyze a series of cryopreserved tissue samples (n = 14), frozen samples that underwent a severe thawing process (n = 10), and their paired formalin-fixed paraffin-embedded (FFPE) tissue samples (n = 24) from patients with breast cancer obtained during primary surgical resection and collected in 2011. Quality and integrity analyses of the total and small fraction of RNA were carried out. Recovery of specific RNA molecules (miRNAs hsa-miR-21, hsa-miR-125b, and hsa-miR-191; snoRNA RNU6B; and mRNAs GAPDH and HPRT1) was also analyzed by quantitative RT-PCR. Our results suggest that visualisation of the small RNA electrophoretic profiles obtained using the Agilent 2100 bioanalyzer makes it possible to differentiate between the three groups of samples (optimally frozen, thawed, and FFPE). We demonstrate that specific miRNA molecules can be similarly recovered from different tissue sample sources, which supports their high degree of stability. We conclude that miRNAs are robustly detected irrespective of the quality of the tissue sample. In this regard, a word of caution should be raised before degraded samples are discarded: although prior quality assessment of the biological material to be analyzed is recommended, our work demonstrates that degraded tissue samples are also suitable for miRNA studies.
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Registered trials
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