ArticlePlanta1990
Monoclonal antibodies detect an auxin-induced conformational change in the maize auxin-binding protein.
Article in Planta, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 83 citations in OpenAlex.
- Auxin homeostasis: new roads in a tight network.Journal of experimental botany · 2025Article
- Article
- The Story of Auxin-Binding Protein 1 (ABP1).Cold Spring Harbor perspectives in biology · 2021Review
- The auxin-binding pocket of auxin-binding protein 1 comprises the highly conserved boxes a and c.Planta · 2009Article
- Immunohistochemical localization of IAA and ABP1 in strawberry shoot apexes during floral induction.Planta · 2005Article
- Role of the plasma membrane H+-ATPase in auxin-induced elongation growth: historical and new aspects.Journal of plant research · 2003Article
- Crystal structure of auxin-binding protein 1 in complex with auxin.The EMBO journal · 2002Article
- Article
- Molecular genetics of auxin and cytokinin.Plant molecular biology · 1994Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monoclonal antibody MAC 256 precipitates specifically the auxin-binding protein (ABP) of maize membranes. Auxin-binding activity was recovered from the immunoprecipitate and MAC 256 can, therefore, bind undenatured, native ABP. A sandwich enzyme-linked immunosorbent assay was used to present native ABP to MAC 256 and under these conditions auxins inhibit antibody binding. Millimolar naphthalene-1-acetic acid completely blocks MAC 256 binding and the characteristics of monoclonal antibody MAC 259 are similar. The ability of a range of auxins and related compounds to displace MAC 256 correlates with the known structure-activity relationships of these compounds in vivo and in binding assays. The results are interpreted in terms of an auxin-induced conformational change in ABP, auxin binding leading to a change in, or concealment of, the epitope of the antibody. The epitope for MAC 256 and 259 lies close to the carboxy terminus of the protein, implying that the part of ABP containing the sequence of amino acids responsible for retention within the endoplasmic reticulum is conformationally active.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.