ArticleAmerican journal of physiology. Renal physiology2014
Transepithelial water and urea permeabilities of isolated perfused Munich-Wistar rat inner medullary thin limbs of Henle's loop.
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 25 citations in OpenAlex.
- Urea Transport Mediated by Membrane Proteins of Non-urea-Transporters.Sub-cellular biochemistry · 2025Review
- Renal cell markers: lighthouses for managing renal diseases.American journal of physiology. Renal physiology · 2021Article
- Expression of SLC4A11 protein in mouse and rat medulla: a candidate transporter involved in outer medullary ammonia recycling.Physiological reports · 2019Article
- Mammalian urine concentration: a review of renal medullary architecture and membrane transporters.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2018Review
- The Kidney in Bardet-Biedl Syndrome: Possible Pathogenesis of Urine Concentrating Defect.Kidney diseases (Basel, Switzerland) · 2017Review
- A computational model for simulating solute transport and oxygen consumption along the nephrons.American journal of physiology. Renal physiology · 2016Article
- Urea transport and clinical potential of urearetics.Current opinion in nephrology and hypertension · 2016Review
- Alternative channels for urea in the inner medulla of the rat kidney.American journal of physiology. Renal physiology · 2015Article
- Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema.Molecular medicine reports · 2015Article
- Architecture of the human renal inner medulla and functional implications.American journal of physiology. Renal physiology · 2015Article
- Isolation and perfusion of rat inner medullary vasa recta.American journal of physiology. Renal physiology · 2015Article
- A mathematical model of rat proximal tubule and loop of Henle.American journal of physiology. Renal physiology · 2015Article
- Urine-concentrating mechanism in the inner medulla: function of the thin limbs of the loops of Henle.Clinical journal of the American Society of Nephrology : CJASN · 2014Article
- Targeted delivery of solutes and oxygen in the renal medulla: role of microvessel architecture.American journal of physiology. Renal physiology · 2014Review
- Comparative physiology and architecture associated with the mammalian urine concentrating mechanism: role of inner medullary water and urea transport pathways in the rodent medulla.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Review
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4 authors at 1 institution in 1 country.
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Abstract
To better understand the role that water and urea fluxes play in the urine concentrating mechanism, we determined transepithelial osmotic water permeability (Pf) and urea permeability (Purea) in isolated perfused Munich-Wistar rat long-loop descending thin limbs (DTLs) and ascending thin limbs (ATLs). Thin limbs were isolated either from 0.5 to 2.5 mm below the outer medulla (upper inner medulla) or from the terminal 2.5 mm of the inner medulla. Segment types were characterized on the basis of structural features and gene expression levels of the water channel aquaporin 1, which was high in the upper DTL (DTLupper), absent in the lower DTL (DTLlower), and absent in ATLs, and the Cl-(1) channel ClCK1, which was absent in DTLs and high in ATLs. DTLupper Pf was high (3,204.5 ± 450.3 μm/s), whereas DTLlower showed very little or no osmotic Pf (207.8 ± 241.3 μm/s). Munich-Wistar rat ATLs have previously been shown to exhibit no Pf. DTLupper Purea was 40.0 ± 7.3 × 10(-5) cm/s and much higher in DTLlower (203.8 ± 30.3 × 10(-5) cm/s), upper ATL (203.8 ± 35.7 × 10(-5) cm/s), and lower ATL (265.1 ± 49.8 × 10(-5) cm/s). Phloretin (0.25 mM) did not reduce DTLupper Purea, suggesting that Purea is not due to urea transporter UT-A2, which is expressed in short-loop DTLs and short portions of some inner medullary DTLs close to the outer medulla. In summary, Purea is similar in all segments having no osmotic Pf but is significantly lower in DTLupper, a segment having high osmotic Pf. These data are inconsistent with the passive mechanism as originally proposed.
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