ArticleMolecular biology of the cell2014
Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 45 citations in OpenAlex.
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Myosin-1C differentially displaces tropomyosin isoforms altering their inhibition of motility.The Journal of biological chemistry · 2024Article
- Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins.Cytoskeleton (Hoboken, N.J.) · 2023Article
- Distinct actin-tropomyosin cofilament populations drive the functional diversification of cytoskeletal myosin motor complexes.iScience · 2022Article
- Actin-tropomyosin distribution in non-muscle cells.Journal of muscle research and cell motility · 2020Review
- Tropomyosin 3.5 protects the F-actin networks required for tissue biomechanical properties.Journal of cell science · 2018Article
- Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer.Biochemical and biophysical research communications · 2018Article
- Distinct sites in tropomyosin specify shared and isoform-specific regulation of myosins II and V.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Tropomyosin Must Interact Weakly with Actin to Effectively Regulate Thin Filament Function.Biophysical journal · 2017Article
- Kinetic signatures of myosin-5B, the motor involved in microvillus inclusion disease.The Journal of biological chemistry · 2017Article
- ER/Golgi trafficking is facilitated by unbranched actin filaments containing Tpm4.2.Cytoskeleton (Hoboken, N.J.) · 2017Article
- A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.PLoS genetics · 2017Article
- Article
- Tropomyosin isoforms bias actin track selection by vertebrate myosin Va.Molecular biology of the cell · 2016Article
- Analysis of biophysical and functional consequences of tropomyosin-fluorescent protein fusions.FEBS letters · 2016Article
- A single-headed fission yeast myosin V transports actin in a tropomyosin-dependent manner.The Journal of cell biology · 2016Article
- Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis.PLoS biology · 2016Article
- Force-producing ADP state of myosin bound to actin.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Load-dependent modulation of non-muscle myosin-2A function by tropomyosin 4.2.Scientific reports · 2016Article
- Evolutionarily conserved sites in yeast tropomyosin function in cell polarity, transport and contractile ring formation.Biology open · 2015Article
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7 authors at 1 institution in 1 country.
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Abstract
A hallmark of class-V myosins is their processivity--the ability to take multiple steps along actin filaments without dissociating. Our previous work suggested, however, that the fission yeast myosin-V (Myo52p) is a nonprocessive motor whose activity is enhanced by tropomyosin (Cdc8p). Here we investigate the molecular mechanism and physiological relevance of tropomyosin-mediated regulation of Myo52p transport, using a combination of in vitro and in vivo approaches. Single molecules of Myo52p, visualized by total internal reflection fluorescence microscopy, moved processively only when Cdc8p was present on actin filaments. Small ensembles of Myo52p bound to a quantum dot, mimicking the number of motors bound to physiological cargo, also required Cdc8p for continuous motion. Although a truncated form of Myo52p that lacked a cargo-binding domain failed to support function in vivo, it still underwent actin-dependent movement to polarized growth sites. This result suggests that truncated Myo52p lacking cargo, or single molecules of wild-type Myo52p with small cargoes, can undergo processive movement along actin-Cdc8p cables in vivo. Our findings outline a mechanism by which tropomyosin facilitates sorting of transport to specific actin tracks within the cell by switching on myosin processivity.
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