ArticlePflugers Archiv : European journal of physiology2014
A splice variant of the two-pore domain potassium channel TREK-1 with only one pore domain reduces the surface expression of full-length TREK-1 channels.
Article in Pflugers Archiv : European journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 25 citations in OpenAlex.
- The bidirectional interplay between RNA processing and mechanotransduction.Cell reports · 2025Review
- Two-Pore-Domain Potassium (KCells · 2021Review
- Intracellular activation of full-length human TREK-1 channel by hypoxia, high lactate, and low pH denotes polymodal integration by ischemic factors.Pflugers Archiv : European journal of physiology · 2021Article
- Review
- Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina.Scientific reports · 2017Article
- Sodium permeable and "hypersensitive" TREK-1 channels cause ventricular tachycardia.EMBO molecular medicine · 2017Article
- Functional mutagenesis screens reveal the 'cap structure' formation in disulfide-bridge free TASK channels.Scientific reports · 2016Article
- Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ.Frontiers in cellular neuroscience · 2016Article
- Alternatively Spliced Human TREK-1 Variants Alter TREK-1 Channel Function and Localization.Biology of reproduction · 2015Article
- The family of K2P channels: salient structural and functional properties.The Journal of physiology · 2015Review
- Much more than a leak: structure and function of K₂p-channels.Pflugers Archiv : European journal of physiology · 2015Review
- The role of protein-protein interactions in the intracellular traffic of the potassium channels TASK-1 and TASK-3.Pflugers Archiv : European journal of physiology · 2015Review
- The potassium current carried by TREK-1 channels in rat cardiac ventricular muscle.Pflugers Archiv : European journal of physiology · 2015Article
- RNA editing in the central cavity as a mechanism to regulate surface expression of the voltage-gated potassium channel Kv1.1.The Journal of biological chemistry · 2014Article
- Article
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9 authors at 2 institutions in 1 country.
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Abstract
We have identified a novel splice variant of the human and rat two-pore domain potassium (K2P) channel TREK-1. The splice variant TREK-1e results from skipping of exon 5, which causes a frame shift in exon 6. The frame shift produces a novel C-terminal amino acid sequence and a premature termination of translation, which leads to a loss of transmembrane domains M3 and M4 and of the second pore domain. RT-PCR experiments revealed a preferential expression of TREK-1e in kidney, adrenal gland, and amygdala. TREK-1e was nonfunctional when expressed in Xenopus oocytes. However, both the surface expression and the current density of full-length TREK-1 were reduced by co-expression of TREK-1e. Live cell imaging in COS-7 cells transfected with GFP-tagged TREK-1e showed that this splice variant was retained in the endoplasmic reticulum (ER). Attachment of the C-terminus of TREK-1e to two different reporter proteins (Kir2.1 and CD8) led to a strong reduction in the surface expression of these fusion proteins. Progressive truncation of the C-terminus of TREK-1e in these reporter constructs revealed a critical region (amino acids 198 to 205) responsible for the intracellular retention. Mutagenesis experiments indicated that amino acids I204 and W205 are key residues mediating the ER retention of TREK-1e. Our results suggest that the TREK-1e splice variant may interfere with the vesicular traffic of full-length TREK-1 channels from the ER to the plasma membrane. Thus, TREK-1e might modulate the copy number of functional TREK-1 channels at the cell surface, providing a novel mechanism for fine tuning of TREK-1 currents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.