ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1993
Abundance and polymorphism of simple repetitive DNA sequences in Bmssica napus L.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Microsatellite marker development, mapping and applications in rice genetics and breeding.Plant molecular biology · 1997Review
- Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5' -anchored simple sequence repeat (SSR) primers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Identification of the most represented repeated motifs in Arabidopsis thaliana microsatellite loci.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus L. (rapeseed).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Inheritance of rapeseed (Brassica napus)-specific RAPD markers and a transgene in the cross B. juncea x (B. juncea x B. napus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1994Article
- (CAC)5 detects DNA fingerprints and sequences homologous to gene transcripts in rice.Biochemical genetics · 1994Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Brassica napus genome has been investigated by DNA fingerprinting with six synthetic oligonucleotide probes complementary to simple repetitive sequences, namely (GATA)4, (GACA)4, (GGAT)4, (CA)8, (CT)8 and (GTG)5. While all sequence motifs were found to be present in the B. napus genome, their organization and abundance varied considerably. Among the investigated probes, (GATA)4 revealed the highest level of intraspecific polymorphism and distinguishes not only between cultivars but even between different individuals belonging to the same cultivar. In contrast, (GTG)5, (GACA)4 and (GGAT)4 produced relatively homogeneous fingerprint patterns throughout different cultivars, while hybridization to (CT)8 and (CA)8 resulted in only a few weak bands superimposed on a smear. The isoschizomeric pair Hpa II and Msp I revealed the presence of methylated cytosines in the vicinity of (GATA)m repeats. The applicability of simple repetitive sequence polymorphisms as molecular markers for Brassica species is discussed.
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Registered trials
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