ArticleBMC genomics2013
The broccoli (Brassica oleracea) phloem tissue proteome.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa.BMC plant biology · 2025Article
- Proteomics of isolated sieve tubes fromProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Unraveling the Roles of Vascular Proteins Using Proteomics.Molecules (Basel, Switzerland) · 2021Review
- The interplay of phloem-mobile signals in plant development and stress response.Bioscience reports · 2020Review
- Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature.International journal of molecular sciences · 2020Article
- Function of Cyclophilin1 as a long-distance signal molecule in the phloem of tomato plants.Journal of experimental botany · 2017Article
- Phloem Proteomics Reveals New Lipid-Binding Proteins with a Putative Role in Lipid-Mediated Signaling.Frontiers in plant science · 2016Article
- Article
- Recent progress in the use of 'omics technologies in brassicaceous vegetables.Frontiers in plant science · 2015Review
- Advances in plant proteomics toward improvement of crop productivity and stress resistancex.Frontiers in plant science · 2015Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe transport of sugars, hormones, amino acids, proteins, sugar alcohols, and other organic compounds from the sites of synthesis to the sites of use or storage occurs through the conducting cells of the phloem. To better understand these processes a comprehensive understanding of the proteins involved is required. While a considerable amount of data has been obtained from proteomic analyses of phloem sap, this has mainly served to identify the soluble proteins that are translocated through the phloem network.
resultsIn order to obtain more comprehensive proteomic data from phloem tissue we developed a simple dissection procedure to isolate phloem tissue from Brassica oleracea. The presence of a high density of phloem sieve elements was confirmed using light microscopy and fluorescently labeled sieve element-specific antibodies. To increase the depth of the proteomic analysis for membrane bound and associated proteins, soluble proteins were extracted first and subsequent extractions were carried out using two different detergents (SDS and CHAPSO). Across all three extractions almost four hundred proteins were identified and each extraction method added to the analysis demonstrating the utility of an approach combining several extraction protocols.
conclusionsThe phloem was found to be enriched in proteins associated with biotic and abiotic stress responses and structural proteins. Subsequent expression analysis identified a number of genes that appear to be expressed exclusively or at very high levels in phloem tissue, including genes that are known to express specifically in the phloem as well as novel phloem genes.
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