ArticlePloS one2013
Multi-input regulation and logic with T7 promoters in cells and cell-free systems.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications.Nature communications · 2026Article
- Development of a CRISPR/Cas9 genome editing toolbox forSynthetic and systems biotechnology · 2025Article
- Automated and Programmable Cell-Free Systems for Scalable Synthetic Biology with a Focus on Biofoundry Integration.Journal of microbiology and biotechnology · 2025Review
- Innovations, Challenges and Future Directions of T7RNA Polymerase in Microbial Cell Factories.ACS synthetic biology · 2025Review
- Regulatory Components for Bacterial Cell-Free Systems Engineering.ACS synthetic biology · 2024Review
- Preparing for the future of precision medicine: synthetic cell drug regulation.Synthetic biology (Oxford, England) · 2024Review
- Synthetic cells in biomedical applications.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Protocell arrays for simultaneous detection of diverse analytes.Nature communications · 2021Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors.Nature communications · 2018Article
- Scaling up genetic circuit design for cellular computing: advances and prospects.Natural computing · 2018Article
- Preservation of protein expression systems at elevated temperatures for portable therapeutic production.Journal of the Royal Society, Interface · 2017Article
- The application of powerful promoters to enhance gene expression in industrial microorganisms.World journal of microbiology & biotechnology · 2017Review
- Cell-Free Synthetic Biology: Engineering Beyond the Cell.Cold Spring Harbor perspectives in biology · 2016Review
- Repressor logic modules assembled by rolling circle amplification platform to construct a set of logic gates.Scientific reports · 2016Article
- Modular, multi-input transcriptional logic gating with orthogonal LacI/GalR family chimeras.ACS synthetic biology · 2014Article
- Synthetic biology outside the cell: linking computational tools to cell-free systems.Frontiers in bioengineering and biotechnology · 2014Review
- Developments in the tools and methodologies of synthetic biology.Frontiers in bioengineering and biotechnology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Engineered gene circuits offer an opportunity to harness biological systems for biotechnological and biomedical applications. However, reliance on native host promoters for the construction of circuit elements, such as logic gates, can make the implementation of predictable, independently functioning circuits difficult. In contrast, T7 promoters offer a simple orthogonal expression system for use in a variety of cellular backgrounds and even in cell-free systems. Here we develop a T7 promoter system that can be regulated by two different transcriptional repressors for the construction of a logic gate that functions in cells and in cell-free systems. We first present LacI repressible T7lacO promoters that are regulated from a distal lac operator site for repression. We next explore the positioning of a tet operator site within the T7lacO framework to create T7 promoters that respond to tet and lac repressors and realize an IMPLIES gate. Finally, we demonstrate that these dual input sensitive promoters function in an E. coli cell-free protein expression system. Our results expand the utility of T7 promoters in cell based as well as cell-free synthetic biology applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.