ArticlePloS one2013
The influence of physical and physiological cues on atomic force microscopy-based cell stiffness assessment.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
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- Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy.International journal of molecular sciences · 2022Review
- The Role of the Dynamic Lung Extracellular Matrix Environment on Fibroblast Morphology and Inflammation.Cells · 2022Article
- The Pathophysiologic Role of Gelsolin in Chronic Kidney Disease: Focus on Podocytes.International journal of molecular sciences · 2021Article
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- Evaluation of quasi-static and dynamic nanomechanical properties of bone-metastatic breast cancer cells using a nanoclay cancer testbed.Scientific reports · 2021Article
- Viscoelasticity and Volume of Cortical Neurons under Glutamate Excitotoxicity and Osmotic Challenges.Biophysical journal · 2020Article
- Single-Cell Elasticity Measurement with an Optically Actuated Microrobot.Micromachines · 2020Article
- Indenting soft samples (hydrogels and cells) with cantilevers possessing various shapes of probing tip.European biophysics journal : EBJ · 2020Article
- Vimentin Plays a Crucial Role in Fibroblast Ageing by Regulating Biophysical Properties and Cell Migration.Cells · 2019Article
- Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts.Journal of the Royal Society, Interface · 2019Article
- Anisotropy vs isotropy in living cell indentation with AFM.Scientific reports · 2019Article
- Ultrastructure and Nanomorphology of the American Mink (Mustela vison) Kidney.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2019Article
- Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method.Computational and mathematical methods in medicine · 2019Article
- Pancreatic stellate cells activated by mutant KRAS-mediated PAI-1 upregulation foster pancreatic cancer progression via IL-8.Theranostics · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy provides a novel technique for differentiating the mechanical properties of various cell types. Cell elasticity is abundantly used to represent the structural strength of cells in different conditions. In this study, we are interested in whether physical or physiological cues affect cell elasticity in Atomic force microscopy (AFM)-based assessments. The physical cues include the geometry of the AFM tips, the indenting force and the operating temperature of the AFM. All of these cues show a significant influence on the cell elasticity assessment. Sharp AFM tips create a two-fold increase in the value of the effective Young's modulus (E(eff)) relative to that of the blunt tips. Higher indenting force at the same loading rate generates higher estimated cell elasticity. Increasing the operation temperature of the AFM leads to decreases in the cell stiffness because the structure of actin filaments becomes disorganized. The physiological cues include the presence of fetal bovine serum or extracellular matrix-coated surfaces, the culture passage number, and the culture density. Both fetal bovine serum and the extracellular matrix are critical for cells to maintain the integrity of actin filaments and consequently exhibit higher elasticity. Unlike primary cells, mouse kidney progenitor cells can be passaged and maintain their morphology and elasticity for a very long period without a senescence phenotype. Finally, cell elasticity increases with increasing culture density only in MDCK epithelial cells. In summary, for researchers who use AFM to assess cell elasticity, our results provide basic and significant information about the suitable selection of physical and physiological cues.
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