ArticleNucleic acids research2014
Polymer modeling of the E. coli genome reveals the involvement of locus positioning and macrodomain structuring for the control of chromosome conformation and segregation.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Physical models of bacterial chromosomes.Molecular microbiology · 2025Review
- Loop-extruders alter bacterial chromosome topology to direct entropic forces for segregation.Nature communications · 2024Article
- Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells.Nature communications · 2024Article
- Chromosome structure modeling tools and their evaluation in bacteria.Briefings in bioinformatics · 2024Review
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- Interpretation of organizational role of proteins on E. coli nucleoid via Hi-C integrated model.Biophysical journal · 2023Article
- DNA supercoiling in bacteria: state of play and challenges from a viewpoint of physics based modeling.Frontiers in microbiology · 2023Review
- Dynamics of chromosome organization in a minimal bacterial cell.Frontiers in cell and developmental biology · 2023Article
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- Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events inMolecular systems biology · 2018Article
- The Origin of Chromosomal Replication Is Asymmetrically Positioned on the Mycobacterial Nucleoid, and the Timing of Its Firing Depends on HupB.Journal of bacteriology · 2018Article
- Review
- Features of genomic organization in a nucleotide-resolution molecular model of the Escherichia coli chromosome.Nucleic acids research · 2017Article
- Horizontally acquired AT-rich genes in Escherichia coli cause toxicity by sequestering RNA polymerase.Nature microbiology · 2017Article
- The MaoP/maoS Site-Specific System Organizes the Ori Region of the E. coli Chromosome into a Macrodomain.PLoS genetics · 2016Article
- Comparative Genomics of Interreplichore Translocations in Bacteria: A Measure of Chromosome Topology?G3 (Bethesda, Md.) · 2016Article
- The role of MatP, ZapA and ZapB in chromosomal organization and dynamics in Escherichia coli.Nucleic acids research · 2016Article
- Assessing the limits of restraint-based 3D modeling of genomes and genomic domains.Nucleic acids research · 2015Article
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3 authors.
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Abstract
The mechanisms that control chromosome conformation and segregation in bacteria have not yet been elucidated. In Escherichia coli, the mere presence of an active process remains an open question. Here, we investigate the conformation and segregation pattern of the E. coli genome by performing numerical simulations on a polymer model of the chromosome. We analyze the roles of the intrinsic structuring of chromosomes and the forced localization of specific loci, which are observed in vivo. Specifically, we examine the segregation pattern of a chromosome that is divided into four structured macrodomains (MDs) and two non-structured regions. We find that strong osmotic-like organizational forces, which stem from the differential condensation levels of the chromosome regions, dictate the cellular disposition of the chromosome. Strikingly, the comparison of our in silico results with fluorescent imaging of the chromosome choreography in vivo reveals that in the presence of MDs the targeting of the origin and terminus regions to specific positions are sufficient to generate a segregation pattern that is indistinguishable from experimentally observed patterns.
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