ArticleMolecular biology and evolution2014
Rapid creation of forward-genetics tools for C. briggsae using TALENs: lessons for nonmodel organisms.
Article in Molecular biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Origins of Self-Fertility in ThreeBiology · 2026Review
- Haldane's law works through X:Autosome incompatibility in Caenorhabditis briggsae/C. nigoni hybrids.Nature communications · 2026Article
- TheiScience · 2025Article
- Article
- Rewiring the Sex-Determination Pathway During the Evolution of Self-Fertility.Molecular biology and evolution · 2024Article
- Orthologs of the Caenorhabditis elegans heterochronic genes have divergent functions in Caenorhabditis briggsae.Genetics · 2023Article
- Efficient targeted recombination with CRISPR/Cas9 in hybrids of Caenorhabditis nematodes with suppressed recombination.BMC biology · 2023Article
- Functional divergence of orthologous temperature-sensitive mutations inmicroPublication biology · 2022Article
- Genome editing in the nematodeBiology methods & protocols · 2020Article
- Article
- From "the Worm" to "the Worms" and Back Again: The Evolutionary Developmental Biology of Nematodes.Genetics · 2018Review
- The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes.PLoS biology · 2018Article
- Revisiting Suppression of Interspecies Hybrid Male Lethality inG3 (Bethesda, Md.) · 2017Article
- Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis.PLoS genetics · 2016Article
- Genome Editing in C. elegans and Other Nematode Species.International journal of molecular sciences · 2016Review
- Suppression of F1 Male-Specific Lethality in Caenorhabditis Hybrids by cbr-him-8.G3 (Bethesda, Md.) · 2015Article
- Rendering the Intractable More Tractable: Tools from Caenorhabditis elegans Ripe for Import into Parasitic Nematodes.Genetics · 2015Review
- Dependence of the sperm/oocyte decision on the nucleosome remodeling factor complex was acquired during recent Caenorhabditis briggsae evolution.Molecular biology and evolution · 2014Article
- Rogue sperm indicate sexually antagonistic coevolution in nematodes.PLoS biology · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Although evolutionary studies of gene function often rely on RNA interference, the ideal approach would use reverse genetics to create null mutations for cross-species comparisons and forward genetics to identify novel genes in each species. We have used transcription activator-like effector nucleases (TALENs) to facilitate both approaches in Caenorhabditis nematodes. First, by combining golden gate cloning and TALEN technology, we can induce frameshifting mutations in any gene. Second, by combining this approach with bioinformatics we can predict and create the resources needed for forward genetic analysis in species like Caenorhabditis briggsae. Although developing genetic model organisms used to require years to isolate marker mutations, balancers, and tools, with TALENs, these reagents can now be produced in months. Furthermore, the analysis of nonsense mutants in related model organisms allows a directed approach for making these markers and tools. When used together, these methods could simplify the adaptation of other organisms for forward and reverse genetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.