ArticleProceedings of the National Academy of Sciences of the United States of America2013
High-speed holographic microscopy of malaria parasites reveals ambidextrous flagellar waveforms.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 78 citations in OpenAlex.
- Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.Small methods · 2026Article
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Bacteria Optimize Tumble Bias to Strategically Navigate Surface Constraints.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Real-time 3D tracking of swimming microbes using digital holographic microscopy and deep learning.PloS one · 2024Article
- FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction.Nature communications · 2023Article
- Fast detection of slender bodies in high density microscopy data.Communications biology · 2023Article
- Plasmodium falciparum Cysteine Rich Secretory Protein uniquely localizes to one end of male gametes.Molecular and biochemical parasitology · 2022Article
- Multifocal imaging for precise, label-free tracking of fast biological processes in 3D.Nature communications · 2021Article
- Structure of the trypanosome paraflagellar rod and insights into non-planar motility of eukaryotic cells.Cell discovery · 2021Article
- Reconstruction of the three-dimensional beat pattern underlying swimming behaviors of sperm.The European physical journal. E, Soft matter · 2021Article
- High-speed, three-dimensional imaging reveals chemotactic behaviour specific to human-infectiveeLife · 2021Article
- Modelling Motility: The Mathematics of Spermatozoa.Frontiers in cell and developmental biology · 2021Review
- High spatial and temporal resolution synthetic aperture phase microscopy.Advanced photonics · 2020Article
- Haloarchaea swim slowly for optimal chemotactic efficiency in low nutrient environments.Nature communications · 2020Article
- Flagellar kinematics reveals the role of environment in shaping sperm motility.Journal of the Royal Society, Interface · 2020Article
- RETRACTED: Human sperm uses asymmetric and anisotropic flagellar controls to regulate swimming symmetry and cell steering.Science advances · 2020Article
- High-resolution 4-D acquisition of freely swimming human sperm cells without staining.Science advances · 2020Article
- Motility Analysis of Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2020Article
- New Twists and Turns in Bacterial Locomotion and Signal Transduction.Journal of bacteriology · 2019Review
- High-speed multifocal plane fluorescence microscopy for three-dimensional visualisation of beating flagella.Journal of cell science · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Axonemes form the core of eukaryotic flagella and cilia, performing tasks ranging from transporting fluid in developing embryos to the propulsion of sperm. Despite their abundance across the eukaryotic domain, the mechanisms that regulate the beating action of axonemes remain unknown. The flagellar waveforms are 3D in general, but current understanding of how axoneme components interact stems from 2D data; comprehensive measurements of flagellar shape are beyond conventional microscopy. Moreover, current flagellar model systems (e.g., sea urchin, human sperm) contain accessory structures that impose mechanical constraints on movement, obscuring the "native" axoneme behavior. We address both problems by developing a high-speed holographic imaging scheme and applying it to the (male) microgametes of malaria (Plasmodium) parasites. These isolated flagella are a unique, mathematically tractable model system for the physics of microswimmers. We reveal the 3D flagellar waveforms of these microorganisms and map the differential shear between microtubules in their axonemes. Furthermore, we overturn claims that chirality in the structure of the axoneme governs the beat pattern [Hirokawa N, et al. (2009) Ann Rev Fluid Mech 41:53-72], because microgametes display a left- or right-handed character on alternate beats. This breaks the link between structural chirality in the axoneme and larger scale symmetry breaking (e.g., in developing embryos), leading us to conclude that accessory structures play a critical role in shaping the flagellar beat.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.