ArticleThe Journal of biological chemistry2013
Catabolite control protein E (CcpE) is a LysR-type transcriptional regulator of tricarboxylic acid cycle activity in Staphylococcus aureus.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- The Transcriptional Regulator Crt1 is Involved in The Pathogenic Lifestyle ofPolish journal of microbiology · 2026Article
- Genome-wide mutagenesis identifies factors involved in MRSA vaginal colonization.Cell reports · 2025Article
- Development and validation of genome-informed and multigene-based qPCR and LAMP assays for accurate detection ofMicrobiology spectrum · 2025Article
- Staphylococcal aconitase expression during iron deficiency is controlled by an sRNA-driven feedforward loop and moonlighting activity.Nucleic acids research · 2024Article
- Citrate serves as a signal molecule to modulate carbon metabolism and iron homeostasis in Staphylococcus aureus.PLoS pathogens · 2024Article
- Molecular mechanism of the one-component regulator RccR on bacterial metabolism and virulence.Nucleic acids research · 2024Article
- First report on in-depth genome and comparative genome analysis of a metal-resistant bacteriumFrontiers in microbiology · 2024Article
- Exploring the targetome of IsrR, an iron-regulated sRNA controlling the synthesis of iron-containing proteins inFrontiers in microbiology · 2024Article
- IL-17A inhibitors alleviate Psoriasis with concomitant restoration of intestinal/skin microbiota homeostasis and altered microbiota function.Frontiers in immunology · 2024Article
- Virulence attributes of successful methicillin-resistantClinical microbiology reviews · 2023Review
- Adaptive Laboratory Evolution ofAntibiotics (Basel, Switzerland) · 2023Article
- Role of sodium salicylate inFrontiers in microbiology · 2022Article
- Functional and structural analysis of catabolite control protein C that responds to citrate.Scientific reports · 2021Article
- Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence inMicroorganisms · 2021Article
- TheFrontiers in microbiology · 2021Article
- Article
- Contributions of a LysR Transcriptional Regulator to Listeria monocytogenes Virulence and Identification of Its Regulons.Journal of bacteriology · 2020Article
- Crosstalk BetweenFrontiers in immunology · 2020Review
- Virulence and Metabolism.Microbiology spectrum · 2019Review
- Article
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16 authors.
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Abstract
The tricarboxylic acid cycle (TCA cycle) is a central metabolic pathway that provides energy, reducing potential, and biosynthetic intermediates. In Staphylococcus aureus, TCA cycle activity is controlled by several regulators (e.g. CcpA, CodY, and RpiRc) in response to the availability of sugars, amino acids, and environmental stress. Developing a bioinformatic search for additional carbon catabolite-responsive regulators in S. aureus, we identified a LysR-type regulator, catabolite control protein E (CcpE), with homology to the Bacillus subtilis CcpC regulator. Inactivation of ccpE in S. aureus strain Newman revealed that CcpE is a positive transcriptional effector of the first two enzymes of the TCA cycle, aconitase (citB) and to a lesser extent citrate synthase (citZ). Consistent with the transcriptional data, aconitase activity dramatically decreased in the ccpE mutant relative to the wild-type strain. The effect of ccpE inactivation on citB transcription and the lesser effect on citZ transcription were also reflected in electrophoretic mobility shift assays where CcpE bound to the citB promoter but not the citZ promoter. Metabolomic studies showed that inactivation of ccpE resulted in increased intracellular concentrations of acetate, citrate, lactate, and alanine, consistent with a redirection of carbon away from the TCA cycle. Taken together, our data suggest that CcpE is a major direct positive regulator of the TCA cycle gene citB.
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