ArticleChemphyschem : a European journal of chemical physics and physical chemistry2014
Localization microscopy using noncovalent fluorogen activation by genetically encoded fluorogen-activating proteins.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 51 citations in OpenAlex.
- Leaflet-specific phospholipid imaging using genetically encoded proximity sensors.Nature chemical biology · 2026Article
- Transient Fluorescence Labeling: Low Affinity-High Benefits.International journal of molecular sciences · 2021Review
- Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy.ACS chemical biology · 2021Article
- Incorporation of sensing modalities into de novo designed fluorescence-activating proteins.Nature communications · 2021Article
- Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells.PloS one · 2021Article
- Near-infrared fluorescent probes: a next-generation tool for protein-labeling applications.Chemical science · 2020Review
- Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking.Journal of visualized experiments : JoVE · 2020Article
- Genetically Encoded Activators of Small Molecules for Imaging and Drug Delivery.Angewandte Chemie (International ed. in English) · 2020Review
- Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging.International journal of molecular sciences · 2019Review
- Single-molecule localization microscopy and tracking with red-shifted states of conventional BODIPY conjugates in living cells.Nature communications · 2019Article
- Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System.ACS chemical biology · 2019Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Translocation of small molecules through engineered outer-membrane channels from Gram-negative bacteria.The European physical journal. E, Soft matter · 2018Article
- Fluorogen-activating proteins: beyond classical fluorescent proteins.Acta pharmaceutica Sinica. B · 2018Review
- Differential mast cell outcomes are sensitive to FcεRI-Syk binding kinetics.Molecular biology of the cell · 2017Article
- Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal.Chemical science · 2017Article
- Fluorogenic Labeling Strategies for Biological Imaging.International journal of molecular sciences · 2017Review
- Versatile Interacting Peptide (VIP) Tags for Labeling Proteins with Bright Chemical Reporters.Chembiochem : a European journal of chemical biology · 2017Article
- Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule.Journal of the American Chemical Society · 2016Article
- Dark dyes-bright complexes: fluorogenic protein labeling.Current opinion in chemical biology · 2015Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
The noncovalent equilibrium activation of a fluorogenic malachite green dye and its cognate fluorogen-activating protein (FAP) can produce a sparse labeling distribution of densely tagged genetically encoded proteins, enabling single molecule detection and super-resolution imaging in fixed and living cells. These sparse labeling conditions are achieved by control of the dye concentration in the milieu, and do not require any photoswitching or photoactivation. The labeling is achieved by using physiological buffers and cellular media, in which additives and switching buffers are not required to obtain super-resolution images. We evaluate the super-resolution properties and images obtained from a selected FAP clone fused to actin, and show that the photon counts per object are between those typically reported for fluorescent proteins and switching-dye pairs, resulting in 10-30 nm localization precision per object. This labeling strategy complements existing approaches, and may simplify multicolor labeling of cellular structures.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.