ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1993
Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- The place of asymmetric somatic hybridization in wheat breeding.Plant cell reports · 2014Review
- Fertile introgression products generated via somatic hybridization between wheat and Thinopyrum intermedium.Plant cell reports · 2014Article
- Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Raphanus sativus).Plant cell reports · 2003Article
- Production of intertribal somatic hybrids between Brassica napus L. and Lesquerella fendleri (Gray) Wats.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Plant regeneration from protoplasts ofVicia narbonensis via somatic embryogenesis and shoot organogenesis.Plant cell reports · 1996Article
- Introduction of a gene from fertility restored radish (Raphanus sativus) into Brassica napus by fusion of X-irradiated protoplasts from a radish restorer line and iodacetoamide-treated protoplasts from a cytoplasmic male-sterile cybrid of B. napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Rare symmetric and asymmetric Nicotiana tabacum (+) N. megalosiphon somatic hybrids recovered by selection for nuclear-encoded resistance genes and in the absence of genome inactivation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1995Article
- Chromosome elimination in asymmetric somatic hybrids: effect of gamma dose and time in culture.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1994Article
- Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell suspension-derived protoplasts of a chlorsulfuron-resistant (GH50) strain of Arabidopsis thaliana cv Columbia were X-irradiated at 60 or 90 krad, to facilitate the elimination of GH50 donor chromosomes in fusion products. Irradiated GH50 protoplasts were fused, with polyethylene glycol, to protoplasts derived from stem epidermal strips of Brassica napus cv Westar. Chlorsulfuron-resistant colonies were selected in vitro and then transferred to shoot and root regeneration medium. Seventeen hybrid lines were regenerated in vitro, and eight were successfully established in the greenhouse, where they flowered. These eight asymmetric hybrids were intermediate in vegetative morphology between Arabidopsis and Brassica. The flowers from these hybrids were male-sterile with abnormal petal and pistil structures. Zymograms for phosphoglucomutase, esterase, and peroxidase showed the presence of all parental isozymes in each of the hybrids tested. Nuclear hybridity was also confirmed for the ribosomal RNA genes using a wheat rDNA probe; however, the chloroplast genome in each of the hybrids was derived solely from the Brassica parent. All selected somatic hybrids were capable of rooting at levels of chlorsulfuron which were inhibitory to unfused Brassica plantlets. The degree of herbicide resistance in the hybrid shoots is presently being evaluated.
Identifiers
24193454What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.