ArticleJournal of visualized experiments : JoVE2013
Collection and analysis of Arabidopsis phloem exudates using the EDTA-facilitated Method.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
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- The role of transcription factor StBEL11 in carbon allocation and tuberization in cultivated potato differs from that known for the model Andean genotype.Journal of experimental botany · 2026Article
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- Laser dissection-assisted phloem transcriptomics highlights the metabolic and physiological changes accompanying clubroot disease progression in oilseed rape.The Plant journal : for cell and molecular biology · 2025Article
- Dual nematode infection in Brassica nigra affects shoot metabolome and aphid survival in distinct contrast to single-species infection.Journal of experimental botany · 2024Article
- Evolutionary and Structural Analysis of PP16 in Viridiplantae.International journal of molecular sciences · 2024Article
- The Small RNA Component ofPlants (Basel, Switzerland) · 2023Article
- Differential Effects of Senescence on the Phloem Exports of Cadmium and Zinc from Leaves to Grains in Rice during Grain Filling.Plants (Basel, Switzerland) · 2023Article
- Arabidopsis ABCG14 forms a homodimeric transporter for multiple cytokinins and mediates long-distance transport of isopentenyladenine-type cytokinins.Plant communications · 2023Article
- A nitrogen fixing symbiosis-specific pathway required for legume flowering.Science advances · 2023Article
- VOZ1 and VOZ2 transcription factors regulate arsenic tolerance and distribution in rice and Arabidopsis.Frontiers in plant science · 2023Article
- Drought and heat stress mediated activation of lipid signaling in plants: a critical review.Frontiers in plant science · 2023Review
- Girdling promotes tomato fruit enlargement by enhancing fruit sink strength and triggering cytokinin accumulation.Frontiers in plant science · 2023Article
- Functional Characterization of anMolecules (Basel, Switzerland) · 2022Article
- Phased small RNA-mediated systemic signaling in plants.Science advances · 2022Article
- Mobile forms of carbon in trees: metabolism and transport.Tree physiology · 2022Review
- The Reciprocal Effect of Elevated COFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant phloem is essential for the long-distance transport of (photo-) assimilates as well as of signals conveying biotic or abiotic stress. It contains sugars, amino acids, proteins, RNA, lipids and other metabolites. While there is a large interest in understanding the composition and function of the phloem, the role of many of these molecules and thus, their importance in plant development and stress response has yet to be determined. One barrier to phloem analysis lies in the fact that the phloem seals itself upon wounding. As a result, the number of plants from which phloem sap can be obtained is limited. One method that allows collection of phloem exudates from several plant species without added equipment is the EDTA-facilitated phloem exudate collection described here. While it is easy to use, it does lead to the wounding of cells and care has to be taken to remove contents of damaged cells. In addition, several controls to prove purity of the exudate are necessary. Because it is an exudation rather than a direct collection of the phloem sap (not possible in many species) only relative quantification of its contents can occur. The advantage of this method over others is that it can be used in many herbaceous or woody plant species (Perilla, Arabidopsis, poplar, etc.) and requires minimal equipment and training. It leads to reasonably large amounts of exudates that can be used for subsequent analysis of proteins, sugars, lipids, RNA, viruses and metabolites. It is simple enough that it can be used in both a research as well as in a teaching laboratory.
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