ArticleJournal of visualized experiments : JoVE2013
High resolution whole mount in situ hybridization within zebrafish embryos to study gene expression and function.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 24 citations in OpenAlex.
- Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.The Journal of cell biology · 2026Article
- Zebrafish casr modulates cardiac structure and function.Scientific reports · 2026Article
- Tead1a Initiates Transcriptional Priming Through the TEAD1a/YAP-Notch1-Spi1/Cebpα Axis to Promote Neutrophil Fate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Functional Characterization of Trypsin in the Induction of Biologically Live Bait Feeding in Mandarin Fish (Cells · 2025Article
- Mutant zebrafish lacking slc25a22a show spontaneous seizures and respond to the anti-seizure medication valproic acid.Disease models & mechanisms · 2025Article
- Knockout ofBiomedicines · 2024Article
- The pathogenic mechanism of syndactyly type V identified in a Hoxd13Q50R knock-in mice.Bone research · 2024Article
- ZebrafishFrontiers in molecular neuroscience · 2024Article
- Mechanical spinal cord transection in larval zebrafish and subsequent whole-mount histological processing.STAR protocols · 2022Article
- Mitochondrial connections with immune system in Zebrafish.Fish and shellfish immunology reports · 2021Review
- Functions ofBiomedicines · 2021Article
- TRIM28 regulates sprouting angiogenesis through VEGFR-DLL4-Notch signaling circuit.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- The NOTCH1-dependent HIF1α/VGLL4/IRF2BP2 oxygen sensing pathway triggers erythropoiesis terminal differentiation.Redox biology · 2020Article
- Knockout of zebrafish interleukin 7 receptor (IL7R) by the CRISPR/Cas9 system delays retinal neurodevelopment.Cell death & disease · 2018Article
- Zebrafish nephrosin helps host defence againstOpen biology · 2017Article
- Down-regulation of interleukin 7 receptor (IL-7R) contributes to central nervous system demyelination.Oncotarget · 2017Article
- Spatiotemporal expression and transcriptional regulation of heme oxygenase and biliverdin reductase genes in zebrafish (Danio rerio) suggest novel roles during early developmental periods of heightened oxidative stress.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2017Article
- Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos.Journal of visualized experiments : JoVE · 2016Article
- An Optimized Small Tissue Handling System for Immunohistochemistry and In Situ Hybridization.PloS one · 2016Article
- Temperature Sensitivity of Neural Tube Defects in Zoep Mutants.Zebrafish · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This article focuses on whole-mount in situ hybridization (WISH) of zebrafish embryos. The WISH technology facilitates the assessment of gene expression both in terms of tissue distribution and developmental stage. Protocols are described for the use of WISH of zebrafish embryos using antisense RNA probes labeled with digoxigenin. Probes are generated by incorporating digoxigenin-linked nucleotides through in vitro transcription of gene templates that have been cloned and linearized. The chorions of embryos harvested at defined developmental stages are removed before incubation with specific probes. Following a washing procedure to remove excess probe, embryos are incubated with anti-digoxigenin antibody conjugated with alkaline phosphatase. By employing a chromogenic substrate for alkaline phosphatase, specific gene expression can be assessed. Depending on the level of gene expression the entire procedure can be completed within 2-3 days.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.