ArticleTicks and tick-borne diseases2014
Single-tube real-time PCR assay for differentiation of Ixodes affinis and Ixodes scapularis.
Article in Ticks and tick-borne diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed.
- A next generation sequencing assay combining Ixodes species identification with pathogen detection to support tick surveillance efforts in the United States.Ticks and tick-borne diseases · 2024Article
- Population genetics of the Ixodes affinis (Ixodida: Ixodidae) complex in America: new findings and a host-parasite review.Parasitology research · 2023Article
- Deer management generally reduces densities of nymphal Ixodes scapularis, but not prevalence of infection with Borrelia burgdorferi sensu stricto.Ticks and tick-borne diseases · 2023Article
- Analysis of macerated ticks using Boolean logic gating colorimetric isothermal nucleic acid assays for Lyme Borrelia and Ixodes scapularis ticks.Scientific reports · 2023Article
- Tick Species Composition, Collection Rates, and Phenology Provide Insights into Tick-Borne Disease Ecology in Virginia.Journal of medical entomology · 2022Article
- Tick species infesting humans in the United States.Ticks and tick-borne diseases · 2022Review
- Focus Stacking Images of Morphological Character States for Differentiating the Adults of Ixodes affinis and Ixodes scapularis (Acari: Ixodidae) in Areas of Sympatry.Journal of medical entomology · 2021Article
- Passerine birds as hosts for Ixodes ticks infected with Borrelia burgdorferi sensu stricto in southeastern Virginia.Ticks and tick-borne diseases · 2021Article
- First report of Candidatus Rickettsia mendelii in Ixodes brunneus from the United States.Ticks and tick-borne diseases · 2020Article
- Identification of closely related Ixodes species by protein profiling with MALDI-TOF mass spectrometry.PloS one · 2019Article
- Borrelia miyamotoi, Other Vector-Borne Agents in Cat Blood and Ticks in Eastern Maryland.EcoHealth · 2017Article
- Prevalence and Diversity of Tick-Borne Pathogens in Nymphal Ixodes scapularis (Acari: Ixodidae) in Eastern National Parks.Journal of medical entomology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Ixodes affinis Neumann (1899) and Ixodes scapularis Say (1821) are tick vectors of the etiologic agent of Lyme disease, Borrelia burgdorferi sensu stricto. Ixodes affinis and I. scapularis are morphologically very similar, and as they are sympatric in the mid- and south-Atlantic U.S. coastal states, their accurate identification is crucial to studies of disease and vector ecology in this area. This work describes a rapid, single-tube SYBR(®) Green-based real-time PCR assay for differentiation of I. affinis and I. scapularis at all life stages. The assay employs 2 pairs of species-specific primers directed against the internal transcribed spacer 2 (ITS2) region of the nuclear rRNA operon. Amplification products for these primer pairs differ in size and may be differentiated with a melt curve analysis. This tool is intended as a supplement to morphological methods for accurate identification of these ticks.
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