ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2014
Serotonergic neuron regulation informed by in vivo single-cell transcriptomics.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Assessing the association between combined oral contraceptive use, selected biomarkers, and depressive episodes among premenopausal women: a protocol for a systematic review and meta-analysis.Systematic reviews · 2026Article
- Roles of synaptic cell adhesion molecules in the neuromodulatory systems.Molecules and cells · 2026Review
- Molecular Organization of Autonomic, Respiratory, and Spinally-Projecting Neurons in the Mouse Ventrolateral Medulla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Optogenetic Stimulation of Midbrain Dopamine Neurons Produces Striatal Serotonin Release.ACS chemical neuroscience · 2022Article
- Spatiotemporal Control of Noradrenaline-Dependent Synaptic Transmission in Mouse Dorsal Raphe Serotonin Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Histamine and histamine receptors: Roles in major depressive disorder.Frontiers in psychiatry · 2022Review
- Pten is a key intrinsic factor regulating raphe 5-HT neuronal plasticity and depressive behaviors in mice.Translational psychiatry · 2021Article
- Cocaine reward and memory after chemogenetic inhibition of distinct serotonin neuron subtypes in mice.Psychopharmacology · 2020Article
- Article
- Serotonergic modulation across sensory modalities.Journal of neurophysiology · 2020Review
- Histamine Receptors Regulate the Activity, Surface Expression, and Phosphorylation of Serotonin Transporters.ACS chemical neuroscience · 2020Article
- Single-cell analysis to understand the diversity of immune cell types that drive disease pathogenesis.The Journal of allergy and clinical immunology · 2019Review
- Article
- Embracing diversity in the 5-HT neuronal system.Nature reviews. Neuroscience · 2019Review
- GPCRomics: An Approach to Discover GPCR Drug Targets.Trends in pharmacological sciences · 2019Review
- The successes and future prospects of the linear antisense RNA amplification methodology.Nature protocols · 2018Article
- Serotonin neuron development: shaping molecular and structural identities.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Putting it in Context: Linking Auditory Processing with Social Behavior Circuits in the Vertebrate Brain.Integrative and comparative biology · 2017Review
- Article
- Single-Cell RNA Sequencing: Unraveling the Brain One Cell at a Time.Trends in molecular medicine · 2017Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Despite the recognized importance of the dorsal raphe (DR) serotonergic (5-HT) nuclei in the pathophysiology of depression and anxiety, the molecular components/putative drug targets expressed by these neurons are poorly characterized. Utilizing the promoter of an ETS domain transcription factor that is a stable marker of 5-HT neurons (Pet-1) to drive 5-HT neuronal expression of YFP, we identified 5-HT neurons in live acute slices. We isolated RNA from single 5-HT neurons in the ventromedial and lateral wings of the DR and performed single-cell RNA-Seq analysis identifying >500 G-protein coupled receptors (GPCRs) including receptors for classical transmitters, lipid signals, and peptides as well as dozens of orphan-GPCRs. Using these data to inform our selection of receptors to assess, we found that oxytocin and lysophosphatidic acid 1 receptors are translated and active in costimulating, with the α1-adrenergic receptor, the firing of DR 5-HT neurons, while the effects of histamine are inhibitory and exerted at H3 histamine receptors. The inhibitory histamine response provides evidence for tonic in vivo histamine inhibition of 5-HT neurons. This study illustrates that unbiased single-cell transcriptomics coupled with functional analyses provides novel insights into how neurons and neuronal systems are regulated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.