ArticleBiofabrication2013
Single-step laser-based fabrication and patterning of cell-encapsulated alginate microbeads.
Article in Biofabrication, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Modeling intratumor heterogeneity in breast cancer.Biofabrication · 2024Review
- Review
- Laser-Induced Forward Transfer (LIFT) based Bioprinting of the Collagen I with Retina Photoreceptor Cells.Manufacturing letters · 2023Article
- Viscoelastic Properties of Bioprinted Alginate Microbeads Compared to Their Bulk Hydrogel Analogs.Journal of biomechanical engineering · 2023Article
- Cancer Cell Direct Bioprinting: A Focused Review.Micromachines · 2021Review
- 3D Bioprinted cancer models: Revolutionizing personalized cancer therapy.Translational oncology · 2021Review
- Laser-based 3D bioprinting for spatial and size control of tumor spheroids and embryoid bodies.Acta biomaterialia · 2019Article
- On-Demand Radial Electrodeposition of Alginate Tubular Structures.ACS biomaterials science & engineering · 2019Article
- Generation of Cost-Effective Paper-Based Tissue Models through Matrix-Assisted Sacrificial 3D Printing.Nano letters · 2019Article
- Bioinks for 3D bioprinting: an overview.Biomaterials science · 2018Review
- Effects of living cells on the bioink printability during laser printing.Biomicrofluidics · 2017Article
- Directed self-assembly software for single cell deposition.International journal of bioprinting · 2017Article
- Microcapsules and 3D customizable shelled microenvironments from laser direct-written microbeads.Biotechnology and bioengineering · 2016Article
- Recent advances in bioprinting and applications for biosensing.Biosensors · 2014Review
- Hydrogel microspheres for osteoarthritis treatment: Fabrication strategies, smart responsiveness, and multimodal therapies.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Alginate can be used to encapsulate mammalian cells and for the slow release of small molecules. Packaging alginate as microbead structures allows customizable delivery for tissue engineering, drug release, or contrast agents for imaging. However, state-of-the-art microbead fabrication has a limited range in achievable bead sizes, and poor control over bead placement, which may be desired to localize cellular signaling or delivery. Herein, we present a novel, laser-based method for single-step fabrication and precise planar placement of alginate microbeads. Our results show that bead size is controllable within 8%, and fabricated microbeads can remain immobilized within 2% of their target placement. Demonstration of this technique using human breast cancer cells shows that cells encapsulated within these microbeads survive at a rate of 89.6%, decreasing to 84.3% after five days in culture. Infusing rhodamine dye into microbeads prior to fluorescent microscopy shows their 3D spheroidal geometry and the ability to sequester small molecules. Microbead fabrication and patterning is compatible with conventional cellular transfer and patterning by laser direct-write, allowing location-based cellular studies. While this method can also be used to fabricate microbeads en masse for collection, the greatest value to tissue engineering and drug delivery studies and applications lies in the pattern registry of printed microbeads.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.