Evidence map›Paper›PMID 24191970›Full record

ArticleEnvironmental microbiology2014

Non-native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stability.

Sarah V Studer, Julia A Schwartzman, Jessica S Ho, Grant D Geske, Helen E Blackwell, Edward G Ruby

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Sarah V Studer *Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison WI 53706.
Julia A Schwartzman *Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison WI 53706.
Jessica S HoDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison WI 53706.
Grant D GeskeDepartment of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison WI 53706.
Helen E BlackwellDepartment of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison WI 53706.
Edward G RubyDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Drive, Madison WI 53706.
University of Wisconsin–Madison · US

Funding

GRADUATE TRAINING IN MOLECULAR BIOSCIENCEST32GM007215 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI HULL, CHRISTINA M · 1985 to 2018
$22.5M
Chemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8M
VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATIONR01RR012294 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI RUBY, EDWARD G · 1996 to 2011
$6.4M
Vibrio fischeri as a Model for Bacterial ColonizationR37AI050661 · NIAID · UNIVERSITY OF HAWAII AT MANOA · PI MARGARET J MC FALL-NGAI, Edward G Ruby · 2018 to 2026
$5.4M
Vibrio fischeri as a model of Bacterial ColonizationR01AI050661 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI MC FALL-NGAI, MARGARET J, RUBY, EDWARD G · 2002 to 2016
$5.0M
Vibrio Virulence Determinants in a Benign ColonizationR01OD011024 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI MC FALL-NGAI, MARGARET J, RUBY, EDWARD G · 2012 to 2020
$3.9M
Resistance and resiliency in a natural host-microbe symbiosisR01GM099507 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI RUBY, EDWARD G · 2012 to 2015
$1.4M
Synthetic Ligands for Modulating Bacterial CommunicationR01GM109403 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 2014 to 2017
$1.2M
Synthetic Ligands for Modulating Bacterial CommunicationR01AI063326 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 2006 to 2010
$1.1M
NCRR NIH HHS R01 RR012294NIAID NIH HHS AI050661NIAID NIH HHS R01 AI050661NIAID NIH HHS R01 AI063326NIAID NIH HHS R37 AI050661NIGMS NIH HHS R01 GM099507NIGMS NIH HHS R01 GM109403NIGMS NIH HHS T32 GM007215NIGMS NIH HHS T32 GM008505NIGMS NIH HHS T32 GM07215NIH HHS R01 OD011024NIH HHS RR12294/OD11024
6 · The paper itself

Abstract

Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR-type N-acyl L-homoserine (AHL) quorum sensing is common in Gram-negative Proteobacteria, and many members of this group have additional quorum-sensing networks. The bioluminescent symbiont Vibrio fischeri encodes two AHL signal synthases: AinS and LuxI. AinS-dependent quorum sensing converges with LuxI-dependent quorum sensing at the LuxR regulatory element. Both AinS- and LuxI-mediated signalling are required for efficient and persistent colonization of the squid host, Euprymna scolopes. The basis of the mutualism is symbiont bioluminescence, which is regulated by both LuxI- and AinS-dependent quorum sensing, and is essential for maintaining a colonization of the host. Here, we used chemical and genetic approaches to probe the dynamics of LuxI- and AinS-mediated regulation of bioluminescence during symbiosis. We demonstrate that both native AHLs and non-native AHL analogues can be used to non-invasively and specifically modulate induction of symbiotic bioluminescence via LuxI-dependent quorum sensing. Our data suggest that the first day of colonization, during which symbiont bioluminescence is induced by LuxIR, is a critical period that determines the stability of the V. fischeri population once symbiosis is established.

Indexed as

Gene Expression Regulation, Bacterial4-ButyrolactoneAliivibrio fischeriAnimalsBacterial ProteinsDecapodiformesLuminescenceQuorum SensingRepressor ProteinsSymbiosisTime FactorsTrans-ActivatorsTranscription Factors4-ButyrolactoneAinS protein, Vibrio fischeriBacterial Proteinshomoserine lactoneLuxI protein, BacteriaLuxR autoinducer binding proteinsRepressor ProteinsTrans-ActivatorsTranscription Factors

Identifiers

PMID24191970
PMCPMC4010582
OpenAlexW1525665006

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.