ReviewGenetics2013
Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9.
Review in Genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 82 citations in OpenAlex.
- High-Throughput Toxicity Screening withEnvironmental science & technology · 2026Review
- TDP-43 proteinopathies and neurodegeneration: insights from Caenorhabditis elegans models.The FEBS journal · 2026Review
- Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematode Steinernema hermaphroditum.Genetics · 2024Article
- Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids.Advances in parasitology · 2024Article
- Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction.Current oncology (Toronto, Ont.) · 2023Review
- Scalable Electrophysiology of Millimeter-Scale Animals with Electrode Devices.BME frontiers · 2023Review
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- From QTL to gene: C. elegans facilitates discoveries of the genetic mechanisms underlying natural variation.Trends in genetics : TIG · 2021Review
- Insulin signaling regulates longevity through protein phosphorylation in Caenorhabditis elegans.Nature communications · 2021Article
- Complementary Approaches with Free-living and Parasitic Nematodes to Understanding Anthelmintic Resistance.Trends in parasitology · 2021Review
- Toward genetic modification of plant-parasitic nematodes: delivery of macromolecules to adults and expression of exogenous mRNA in second stage juveniles.G3 (Bethesda, Md.) · 2021Article
- Genome editing in the nematodeBiology methods & protocols · 2020Article
- CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases.Biological procedures online · 2020Review
- Electrophysiological Measures of Aging Pharynx Function in C. elegans Reveal Enhanced Organ Functionality in Older, Long-lived Mutants.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- In vivo measurements reveal a single 5'-intron is sufficient to increase protein expression level in Caenorhabditis elegans.Scientific reports · 2019Article
- Article
- A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone.BMC molecular biology · 2018Article
- A toolkit for DNA assembly, genome engineering and multicolor imaging forTranslational medicine of aging · 2018Article
- Highly Efficient, Rapid and Co-CRISPR-Independent Genome Editing inG3 (Bethesda, Md.) · 2017Article
- Reliable CRISPR/Cas9 Genome Engineering inG3 (Bethesda, Md.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 2 countries.
Funding
Abstract
With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand DNA breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed by eight different research groups, six of which are published in GENETICS, to modify the Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering tool levels the playing field for experimental geneticists.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.