ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1994
Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 63 citations in OpenAlex.
- An alginate-layer technique for culture of Brassica oleracea L. protoplasts.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association · 2012Article
- Effect of flavonoids on feeding preference and development of the crucifer pest Mamestra configurata Walker.Journal of chemical ecology · 2004Article
- Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Expression of the C3-C 4 intermediate character in somatic hybrids between Brassica napus and the C3-C 4 species Moricandia arvensis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Production of intertribal somatic hybrids between Brassica napus L. and Lesquerella fendleri (Gray) Wats.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Bromodeoxy uridine combined with UV light and gamma irradiation promotes the production of asymmetric somatic hybrid calli.Plant cell reports · 1996Article
- Transfer of Brassica tournefartii (TT) genes to allotetraploid oilseed Brassica species (B. juncea AABB, B. napus AACC, B. carinata BBCC): homoeologous pairing is more pronounced in the three-genome hybrids (TACC, TBAA, TCAA, TCBB) as compared to allodiploids (TA, TB, TC).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Phylogenetic relationships of Thlaspi s.l. (subtribe Thlaspidinae, Lepidieae) and allied genera based on chloroplast DNA restriction-site variation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- Intertribal somatic hybrids between Brassica napus and Barbarea vulgaris - production of in vitro plantlets.Plant cell reports · 1994Article
- A high frequency of intergenomic mitochondrial recombination and an overall biased segregation of B. campestris or recombined B. campestris mitochondria were found in somatic hybrids made within Brassicaceae.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1994Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protoplast fusions were performed between hypocotyl protoplasts of Brassica napus and mesophyll protoplasts of Thlaspi perfoliatum. The two species are members of the Lepidieae and Brassiceae tribes, respectively, in the family of Brassicaceae. Seeds of T. perfoliatum are rich in the fatty acid C24∶1 (nervonic acid), an oil valuable for technical purposes. In the search for renewable oils to replace the mineral oils, plant breeders have been trying to develop oil crops with a high content of long-chain fatty acids. After fusion of B. napus protoplasts with non-irradiated as well as irradiated protoplasts of T. perfoliatum selection was carried out by flow cytometry and cell sorting. Of the shoots regenerated from different calli 27 were verified as hybrids or partial hybrids using the isoenzyme phosphoglucose isomerase (PGI) as a marker. Another 6 plants were identified as partial hybrids using a T. perfoliatum-specific repetitive DNA sequence. Slot blot experiments were performed to estimate the copy number of the repetitive DNA sequence in the parental species and in the hybrids. In T. perfoliatum there were approximately 10(5) copies per haploid genome, and the range in the hybrids was 1-37% of the value in T. perfoliatum. When the nuclear DNA content of the regenerated shoots was analysed we found partial as well as symmetric hybrids. Even though the rooting and establishment of hybrid shoots in the greenhouse were difficult, resulting in the death of many plants, 19 plants were cultured to full maturity. Seeds obtained from 15 plants were analysed to determine whether they contained nervonic acid, and 5 of the hybrids were found to contain significantly greater amounts of nervonic acid than B. napus.
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Registered trials
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