ArticleScientific reports2013
Production of Sry knockout mouse using TALEN via oocyte injection.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 67 citations in OpenAlex.
- Quantitative dissection of sexual dimorphism in mice through Y-linked gene knockouts and multivariate phenotyping.Scientific reports · 2026Article
- Return of the forgotten hero: the role of Y chromosome-encoded Zfy in male reproduction.Molecular human reproduction · 2023Article
- Substrain differences in Sry expression at the stage of sex determination in C57BL/6 mouse strains.The Journal of veterinary medical science · 2023Article
- Loss of circSRY reduces γH2AX level in germ cells and impairs mouse spermatogenesis.Life science alliance · 2023Article
- First sex modification case in equine cloning.PloS one · 2023Article
- Two acquired mouse Y chromosome-linked genes, Prssly and Teyorf1, are dispensable for male fertility‡.Biology of reproduction · 2022Article
- Review
- Efficient TALEN-mediated gene knockin at the bovine Y chromosome and generation of a sex-reversal bovine.Cellular and molecular life sciences : CMLS · 2021Article
- Alternate Roles of Sox Transcription Factors beyond Transcription Initiation.International journal of molecular sciences · 2021Review
- Mini-Review Regarding the Applicability of Genome Editing Techniques Developed for Studying Infertility.Diagnostics (Basel, Switzerland) · 2021Review
- Knockout of the HMG domain of the porcine SRY gene causes sex reversal in gene-edited pigs.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Sex Manipulation Technologies Progress in Livestock: A Review.Frontiers in veterinary science · 2020Review
- Analysis of the Mouse Y Chromosome by Single-Molecule Sequencing With Y Chromosome Enrichment.Frontiers in genetics · 2020Article
- Efficient production and transmission of CRISPR/Cas9-mediated mutant alleles at the IG-DMR via generation of mosaic mice using a modified 2CC method.Scientific reports · 2019Article
- XY oocytes of sex-reversed females with a Sry mutation deviate from the normal developmental process beyond the mitotic stage†.Biology of reproduction · 2019Article
- Mapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus.Scientific reports · 2018Article
- Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular development.Scientific reports · 2018Article
- CRISPR/Cas9-mediated knock-in of the murine Y chromosomal Sry gene.The Journal of reproduction and development · 2018Article
- One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs.Cell research · 2017Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently developed transcription activator-like effector nuclease (TALEN) technology has enabled the creation of knockout mice, even for genes on the Y chromosome. In this study, we generated a knockout mouse for Sry, a sex-determining gene on the Y chromosome, using microinjection of TALEN RNA into pronuclear stage oocytes. As expected, the knockout mouse had female external and internal genitalia, a female level of blood testosterone and a female sexually dimorphic nucleus in the brain. The knockout mouse exhibited an estrous cycle and performed copulatory behavior as females, although it was infertile or had reduced fertility. A histological analysis showed that the ovary of the knockout mouse displayed a reduced number of oocytes and luteinized unruptured follicles, implying that a reduced number of ovulated oocytes is a possible reason for infertility and/or reduced fertility in the KO mouse.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.