ArticleJournal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract2014
Development and characterization of a surgical mouse model of reflux esophagitis and Barrett's esophagus.
Article in Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Esophageal adenocarcinoma models: a closer look.Frontiers in molecular biosciences · 2024Review
- Activin A-mediated epithelial de-differentiation contributes to injury repair in an in vitro gastrointestinal reflux model.Cytokine · 2019Article
- Cellular Origins of Barrett's Esophagus: the Search Continues.Current gastroenterology reports · 2018Review
- A Summary of the 2016 James W. Freston Conference of the American Gastroenterological Association: Intestinal Metaplasia in the Esophagus and Stomach: Origins, Differences, Similarities and Significance.Gastroenterology · 2017Article
- Columnar Metaplasia in Three Types of Surgical Mouse Models of Esophageal Reflux.Cellular and molecular gastroenterology and hepatology · 2017Article
- Of Mice and Men and Metaplasia.Cellular and molecular gastroenterology and hepatology · 2017Article
- Chemopreventive Strategies for Inflammation-Related Carcinogenesis: Current Status and Future Direction.International journal of molecular sciences · 2017Review
- Deoxycholic acid (DCA) confers an intestinal phenotype on esophageal squamous epithelium via induction of the stemness-associated reprogramming factors OCT4 and SOX2.Cell cycle (Georgetown, Tex.) · 2016Article
- Animal Models of Barrett's Esophagus and Esophageal Adenocarcinoma-Past, Present, and Future.Clinical and translational science · 2015Review
- Activin a signaling regulates cell invasion and proliferation in esophageal adenocarcinoma.Oncotarget · 2015Article
- Esophageal Cancer: Insights From Mouse Models.Cancer growth and metastasis · 2015Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ideally, an animal model of Barrett's esophagus should recapitulate the human disease histologically and immunohistochemically, and be readily susceptible to genetic manipulation. We have developed such a model using a strain of mice commonly used for transgenic and knockout manipulations. We induced reflux by esophagojejunostomy (EJ) in 20 C57Bl/6 mice. At defined time points, sections of the esophagus were stained with H&E and Alcian blue, and immunohistochemical staining was performed for Sox9 (a transcription factor in Barrett's metaplasia), cytokeratin (CK) 8/18 (a columnar marker) and CK14 (a squamous marker). Procedural mortality was 40% for the first ten animals, 20% for the next 10. Reflux esophagitis developed by 13 weeks, and intestinal metaplasia with goblet cells developed by 34 weeks. The metaplasia expressed CK8/18, but not CK14, and exhibited nuclear immunostaining for Sox9. Nuclear Sox9 was also seen in scattered basal cells of squamous epithelium close to the EJ anastomosis. EJ can be performed successfully in C57Bl/6 mice, resulting in reflux esophagitis and intestinal metaplasia that exhibits phenotypic and molecular features of human Barrett's metaplasia. This surgical model in a mouse strain that is easy to manipulate genetically should be a valuable tool for studying the pathogenesis of Barrett's esophagus.
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