ReviewActa crystallographica. Section D, Biological crystallography2013
From lows to highs: using low-resolution models to phase X-ray data.
Review in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Integrative Approaches to Study Virus Structures.Sub-cellular biochemistry · 2024Review
- Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation.Acta crystallographica. Section D, Structural biology · 2021Article
- Using a partial atomic model from medium-resolution cryo-EM to solve a large crystal structure.Acta crystallographica. Section D, Structural biology · 2021Article
- How cryo-electron microscopy and X-ray crystallography complement each other.Protein science : a publication of the Protein Society · 2017Review
- An improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data.Protein & cell · 2015Article
- X-ray structure determination using low-resolution electron microscopy maps for molecular replacement.Nature protocols · 2015Article
- Molecular replacement then and now.Acta crystallographica. Section D, Biological crystallography · 2013Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The study of virus structures has contributed to methodological advances in structural biology that are generally applicable (molecular replacement and noncrystallographic symmetry are just two of the best known examples). Moreover, structural virology has been instrumental in forging the more general concept of exploiting phase information derived from multiple structural techniques. This hybridization of structural methods, primarily electron microscopy (EM) and X-ray crystallography, but also small-angle X-ray scattering (SAXS) and nuclear magnetic resonance (NMR) spectroscopy, is central to integrative structural biology. Here, the interplay of X-ray crystallography and EM is illustrated through the example of the structural determination of the marine lipid-containing bacteriophage PM2. Molecular replacement starting from an ~13 Å cryo-EM reconstruction, followed by cycling density averaging, phase extension and solvent flattening, gave the X-ray structure of the intact virus at 7 Å resolution This in turn served as a bridge to phase, to 2.5 Å resolution, data from twinned crystals of the major coat protein (P2), ultimately yielding a quasi-atomic model of the particle, which provided significant insights into virus evolution and viral membrane biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.