ArticlePathogens and global health2013
Human cytomegalovirus detection by real-time PCR and pp65-antigen test in hematopoietic stem cell transplant recipients: a challenge in low and middle-income countries.
Article in Pathogens and global health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Serum IgG Profiling of Toddlers Reveals a Subgroup with Elevated Seropositive Antibodies to Viruses Correlating with Increased Vaccine and Autoantigen Responses.Journal of clinical immunology · 2021Article
- Plasma cytomegalovirus DNA load predicts outcomes in liver transplant recipients.Immunity, inflammation and disease · 2021Article
- Survey of HCMV in allogenic and autologous stem cell transplantation by real-time PCR in Kermanshah, west of Iran.Infectious agents and cancer · 2021Article
- Cytomegalovirus Cholangiopathy in an Immunocompetent Patient: A Case Report and Literature Review.Case reports in hepatology · 2021Article
- Prevention and Management of CMV Infections after Liver Transplantation: Current Practice in German Transplant Centers.Journal of clinical medicine · 2020Article
- A Jurkat 76 based triple parameter reporter system to evaluate TCR functions and adoptive T cell strategies.Oncotarget · 2018Article
- Detection of Cytomegalovirus Antibodies Using a Biosensor Based on Imaging Ellipsometry.PloS one · 2015Article
- Post liver transplant recurrent and de novo viral infections.Best practice & research. Clinical gastroenterologyReview
- Clinical correlates of pp65 antigenemia monitoring in the first months of post kidney transplant in patients undergoing universal prophylaxis or preemptive therapy.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesObservational
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8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimCytomegalovirus (CMV) infection is one of the most common complications in patients submitted to hematopoietic stem cell transplant (HSCT). Pre-emptive therapy has been indicated in patients with laboratory evidence of CMV replication. The aims of this study were to compare real-time PCR or pp65 antigen assay methodologies to detect CMV replication in HSCT patients, define a viral load threshold for initiation of pre-emptive therapy, and assess the feasibility of its implementation in hospitals of countries with low and middle income. MATERIAL AND
methodsHuman CMV detection by real-time PCR and pp65 antigen assay was carried out in blood and plasma samples of HSCT patients collected weekly during 3 months. Pre-emptive therapy was based on CMV antigenemia results.
resultsTwenty-one patients were monitored with a total of 227 samples collected; 13 (62%) patients were children. A poor correlation was observed between qualitative results, though quantitative results showed statistically significant difference, with higher viral loads detected in patients with positive antigenemia. Compared to a positive antigenemia, a cutoff value of 1067·5 copies/ml, 3·03 log10/ml, for viral load was obtained with 100% sensitivity and 71% specificity.
conclusionCMV real-time PCR in whole blood was suitable for monitoring HSCT patients. However, its high cost is a limiting factor, and it could be used to monitor selected patients, those with prolonged leukopenia and underweight children, and subsequently switched to pp65 antigen test. Further studies involving larger numbers of patients should be performed to confirm this statement.
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