ArticleACS synthetic biology2014
Synthetic core promoters for Pichia pastoris.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 106 citations in OpenAlex.
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- Next-generation stress-inducible Komagataella phaffii promoter variants.Microbial cell factories · 2025Article
- Promoter engineering for enhanced 3-hydroxypropionic acid production inSynthetic and systems biotechnology · 2025Article
- Systematic representation and optimization enable the inverse design of cross-species regulatory sequences in bacteria.Nature communications · 2025Article
- Rational design and characterization of enhanced alcohol-inducible synthetic promoters inApplied and environmental microbiology · 2025Article
- Engineering artificial cross-species promoters with different transcriptional strengths.Synthetic and systems biotechnology · 2025Article
- Designing strong inducible synthetic promoters in yeasts.Nature communications · 2024Article
- Recent progress on heterologous protein production in methylotrophic yeast systems.World journal of microbiology & biotechnology · 2024Review
- Promoters in Pichia pastoris: A Toolbox for Fine-Tuned Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression inMicroorganisms · 2023Article
- Genetic tools for metabolic engineering ofEngineering microbiology · 2023Review
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- An improved CRISPRi system inSynthetic and systems biotechnology · 2023Article
- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Improved Production ofCurrent issues in molecular biology · 2021Article
- Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family.Applied microbiology and biotechnology · 2021Article
- Review
- Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris.Nucleic acids research · 2021Article
- Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris.Microbial cell factories · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostly been focused on altering upstream regulatory sequences such as transcription factor binding sites. In higher eukaryotes synthetic core promoters, directly needed for transcription initiation by RNA Polymerase II, have been successfully designed. Here we report the first synthetic yeast core promoter for P. pastoris, based on natural yeast core promoters. Furthermore we used this synthetic core promoter sequence to engineer the core promoter of the natural AOX1 promoter, thereby creating a set of core promoters providing a range of different expression levels. As opposed to engineering strategies of the significantly longer entire promoter, such short core promoters can directly be added on a PCR primer facilitating library generation and are sufficient to obtain variable expression yields.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.