ArticleThe Journal of biological chemistry2013
Screening for hydrolytic enzymes reveals Ayr1p as a novel triacylglycerol lipase in Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 48 citations in OpenAlex.
- Flexible Cooperation Between Peroxisomes and the Endoplasmic Reticulum During Lipid Synthesis ofCells · 2026Article
- Investigating the effects of membrane curvature inducing proteins on lipid droplets in Saccharomyces cerevisiae.Molecular biology reports · 2025Article
- Nitrogen signaling factor triggers a respiration-like gene expression program in fission yeast.The EMBO journal · 2024Article
- Systematic multi-level analysis of an organelle proteome reveals new peroxisomal functions.Molecular systems biology · 2022Article
- Crystal Structures of the Plant Phospholipase A1 Proteins Reveal a Unique Dimerization Domain.Molecules (Basel, Switzerland) · 2022Article
- The Diversity of the Mitochondrial Outer Membrane Protein Import Channels: Emerging Targets for Modulation.Molecules (Basel, Switzerland) · 2021Review
- Phosphatidic acid biosynthesis in the model organism yeast Saccharomyces cerevisiae - a survey.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- Analysis of autophagy activated during changes in carbon source availability in yeast cells.The Journal of biological chemistry · 2019Article
- Metabolic engineering ofMetabolic engineering communications · 2018Article
- Lipid droplet-mediated lipid and protein homeostasis in budding yeast.FEBS letters · 2018Review
- Identification of new channels by systematic analysis of the mitochondrial outer membrane.The Journal of cell biology · 2017Article
- Analysis of the lipid body proteome of the oleaginous alga Lobosphaera incisa.BMC plant biology · 2017Article
- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe.Journal of microbiology (Seoul, Korea) · 2017Article
- Ymr210wp leads to the accumulation of phospholipids and steryl esters in yeast.Bioinformation · 2017Article
- Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase.Bioscience reports · 2016Article
- An Energy-Independent Pro-longevity Function of Triacylglycerol in Yeast.PLoS genetics · 2016Article
- Lipid droplets and their component triglycerides and steryl esters regulate autophagosome biogenesis.The EMBO journal · 2015Article
- Lipid droplet dynamics in budding yeast.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- Modifications of the C terminus affect functionality and stability of yeast triacylglycerol lipase Tgl3p.The Journal of biological chemistry · 2014Article
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9 authors at 3 institutions in 1 country.
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Abstract
Saccharomyces cerevisiae, as well as other eukaryotes, preserves fatty acids and sterols in a biologically inert form, as triacylglycerols and steryl esters. The major triacylglycerol lipases of the yeast S. cerevisiae identified so far are Tgl3p, Tgl4p, and Tgl5p (Athenstaedt, K., and Daum, G. (2003) YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae. J. Biol. Chem. 278, 23317-23323; Athenstaedt, K., and Daum, G. (2005) Tgl4p and Tgl5p, two triacylglycerol lipases of the yeast Saccharomyces cerevisiae, are localized to lipid particles. J. Biol. Chem. 280, 37301-37309). We observed that upon cultivation on oleic acid, triacylglycerol mobilization did not come to a halt in a yeast strain deficient in all currently known triacylglycerol lipases, indicating the presence of additional not yet characterized lipases/esterases. Functional proteome analysis using lipase and esterase inhibitors revealed a subset of candidate genes for yet unknown hydrolytic enzymes on peroxisomes and lipid droplets. Based on the conserved GXSXG lipase motif, putative functions, and subcellular localizations, a selected number of candidates were characterized by enzyme assays in vitro, gene expression analysis, non-polar lipid analysis, and in vivo triacylglycerol mobilization assays. These investigations led to the identification of Ayr1p as a novel triacylglycerol lipase of yeast lipid droplets and confirmed the hydrolytic potential of the peroxisomal Lpx1p in vivo. Based on these results, we discuss a possible link between lipid storage, lipid mobilization, and peroxisomal utilization of fatty acids as a carbon source.
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