ArticleJournal of bacteriology2014
Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 51 citations in OpenAlex.
- AdiY acts as a cytoplasmic pH sensor via histidine protonation to regulate acid stress adaptation inJournal of bacteriology · 2026Article
- Stigmurin derivatives as potent-biofilm eradicating agents against the major human opportunistic pathogenFrontiers in microbiology · 2026Article
- Squalamine and claramine A1 disperseBiofilm · 2025Article
- Water-Soluble Sulfur-Ylide-Functionalized Polyacrylamides for Antibacterial Surface Applications.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Membrane fluidity homeostasis is required for tobramycin-enhanced biofilm inMicrobiology spectrum · 2024Article
- Transcriptional Regulators Controlling Virulence inInternational journal of molecular sciences · 2023Review
- High affinity iron uptake by pyoverdine in Pseudomonas aeruginosa involves multiple regulators besides Fur, PvdS, and FpvI.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Review
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- The Effects of Sub-inhibitory Antibiotic Concentrations onFrontiers in microbiology · 2021Article
- Rewiring the specificity of extracytoplasmic function sigma factors.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- TacklingBiomolecules · 2020Article
- Activation of the Cell Wall Stress Response inMicroorganisms · 2020Article
- Multidrug Adaptive Resistance of Pseudomonas aeruginosa Swarming Cells.Antimicrobial agents and chemotherapy · 2020Article
- The Temperature-Regulation ofFrontiers in microbiology · 2020Article
- Parallel evolutionary paths to produce more than oneNPJ biofilms and microbiomes · 2020Article
- Membrane-Interactive Compounds FromFrontiers in microbiology · 2020Article
- Article
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa.Scientific reports · 2018Article
- Antisense transcription in Pseudomonas aeruginosa.Microbiology (Reading, England) · 2018Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is distinguished by its broad metabolic diversity and its remarkable capability for adaptation, which relies on a large collection of transcriptional regulators and alternative sigma (σ) factors. The largest group of alternative σ factors is that of the extracytoplasmic function (ECF) σ factors, which control key transduction pathways for maintenance of envelope homeostasis in response to external stress and cell growth. In addition, there are specific roles of alternative σ factors in regulating the expression of virulence and virulence-associated genes. Here, we analyzed a deletion mutant of the ECF σ factor SigX and applied mRNA profiling to define the SigX-dependent regulon in P. aeruginosa in response to low-osmolarity-medium conditions. Furthermore, the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) led to the identification of the DNA binding motif of SigX. Genome-wide mapping of SigX-binding regions revealed enrichment of downstream genes involved in fatty acid biosynthesis, type III secretion, swarming and cyclic di-GMP (c-di-GMP) signaling. In accordance, a sigX deletion mutant exhibited altered fatty acid composition of the cell membrane, reduced cytotoxicity, impaired swarming activity, elevated c-di-GMP levels, and increased biofilm formation. In conclusion, a combination of ChIP-seq with transcriptional profiling and bioinformatic approaches to define consensus DNA binding sequences proved to be effective for the elucidation of the regulon of the alternative σ factor SigX, revealing its role in complex virulence-associated phenotypes in P. aeruginosa.
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