ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik1994
Physical mapping of rRNA genes by fluorescent in-situ hybridization and structural analysis of 5S rRNA genes and intergenic spacer sequences in sugar beet (Beta vulgaris).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 154 citations in OpenAlex.
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- BeetRepeats: reference sequences for genome and polymorphism annotation in sugar beet and wild relatives.BMC research notes · 2024Article
- Cyto-molecular characterization of rDNA and chromatin composition in the NOR-associated satellite in Chestnut (Castanea spp.).Scientific reports · 2024Article
- Sex-determining region complements traditionally used in phylogenetic studies nuclear and chloroplast sequences in investigation ofFrontiers in plant science · 2023Article
- Analysis of 5S rDNA Genomic Organization Through the RepeatExplorer2 Pipeline: A Simplified Protocol.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The Use of Ribosomal DNA for Comparative Cytogenetics.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chorus2: design of genome-scale oligonucleotide-based probes for fluorescence in situ hybridization.Plant biotechnology journal · 2021Article
- Broken, silent, and in hiding: tamed endogenous pararetroviruses escape elimination from the genome of sugar beet (Beta vulgaris).Annals of botany · 2021Article
- Comparative Repeat Profiling of Two Closely Related Conifers (Frontiers in genetics · 2021Article
- The Cassandra retrotransposon landscape in sugar beet (Beta vulgaris) and related Amaranthaceae: recombination and re-shuffling lead to a high structural variability.Annals of botany · 2021Article
- Genomes of the wild beets Beta patula and Beta vulgaris ssp. maritima.The Plant journal : for cell and molecular biology · 2019Article
- Molecular cytogenetic characterization and comparison of the two cultivatedComparative cytogenetics · 2017Article
- Repeat Composition of CenH3-chromatin and H3K9me2-marked heterochromatin in Sugar Beet (Beta vulgaris).BMC plant biology · 2016Article
- Next-generation sequencing reveals differentially amplified tandem repeats as a major genome component of Northern Europe's oldest Camellia japonica.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- An easy "SteamDrop" method for high quality plant chromosome preparation.Molecular cytogenetics · 2014Article
- Combining FISH and model-based predictions to understand chromosome evolution in Typhonium (Araceae).Annals of botany · 2014Article
- Comparative molecular cytogenetic analyses of a major tandemly repeated DNA family and retrotransposon sequences in cultivated jute Corchorus species (Malvaceae).Annals of botany · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A digoxigenin-labelled 5S rDNA probe (pTa-794) and a rhodamine-labelled 18S-5.8S-25S rDNA probe (pTa71) were used for double-target in-situ hybridization to root-tip metaphase, prophase and interphase chromosomes of cultivated beet,Beta vulgaris L. After in-situ hybridization with the 18S-5.8S-25S rDNA probe, one major pair of sites was detected which corresponded to the secondary constriction at the end of the short arm of chromosome 1. The two rDNA chromosomes were often associated and the loci only contracted in late metaphase. In the majority of the metaphase plates analyzed, we found a single additional minor hybridization site with pTa71. One pair of 5S rRNA gene clusters was localized near the centromere on the short arm of one of the three largest chromosomes which does not carry the 18S-5.8S-25S genes. Because of the difficulties in distinguishing the very similarly-sizedB. vulgaris chromosomes in metaphase preparations, the 5S and the 18S-5.8S-25S rRNA genes can be used as markers for chromosome identification. TwoXbaI fragments (pXV1 and pXV2), comprising the 5S ribosomal RNA gene and the adjacent intergenic spacer, were isolated. The two 5S rDNA repeats were 349 bp and 351 bp long, showing considerable sequence variation in the intergenic spacer. The use of fluorescent in-situ hybridization, complemented by molecular data, for gene mapping and for integrating genetic and physical maps of beet species is discussed.
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Registered trials
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