ArticleApplied and environmental microbiology2014
Genomic investigation of lysogen formation and host lysis systems of the Salmonella temperate bacteriophage SPN9CC.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- A temperate phage encoding a catalytically active endolysin: characterization of phage P7869 and its Lys7869 against Pseudomonas aeruginosa.BMC microbiology · 2026Article
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- Analysis of the cell wall binding domain in bacteriocin-like lysin LysL from Lactococcus lactis LAC460.Archives of microbiology · 2024Article
- Biological characterization and genomic analysis of a novel methicillin-resistantMicrobiology spectrum · 2024Article
- Characterization of novel recombinant mycobacteriophages derived from homologous recombination between two temperate phages.G3 (Bethesda, Md.) · 2023Article
- The emerging role of recombineering in microbiology.Engineering microbiology · 2023Review
- Isolation, characterization and whole genome analysis of the novel genus Lederbergvirus, phage vB_EcoP_E212 infecting enterotoxigenic Escherichia coli K88.Virus research · 2023Article
- Genomic analysis of Anderson typing phages of Salmonella Typhimrium: towards understanding the basis of bacteria-phage interaction.Scientific reports · 2023Article
- Phage engineering and phage-assisted CRISPR-Cas delivery to combat multidrug-resistant pathogens.Bioengineering & translational medicine · 2023Review
- CRISPR-Cas9 Based Bacteriophage Genome Editing.Microbiology spectrum · 2022Article
- The Structure and Function of Biomaterial Endolysin EFm1 fromMaterials (Basel, Switzerland) · 2022Article
- Engineered Bacteriophage Therapeutics: Rationale, Challenges and Future.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Diversity and characterization of temperate bacteriophages induced in Pasteurella multocida from different host species.BMC microbiology · 2021Article
- CRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering.Scientific reports · 2021Article
- Phage Therapy for Mycobacterium Abscessus and Strategies to Improve Outcomes.Microorganisms · 2021Review
- JD419, aFrontiers in microbiology · 2021Article
- SGP-C: A Broad Host Range Temperate Bacteriophage; AgainstFrontiers in microbiology · 2021Article
- Exploring the feasibility ofJournal of animal science and technology · 2020Article
- Combined Use of the Ab105-2φΔCI Lytic Mutant Phage and Different Antibiotics in Clinical Isolates of Multi-ResistantMicroorganisms · 2019Article
- Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection.Journal of microbiology (Seoul, Korea) · 2019Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and forms clear plaques with cloudy centers due to lysogen formation. Phylogenetic analysis of phage major capsid proteins revealed that this phage is a member of the lysogen-forming P22-like phage group. However, comparative genomic analysis of SPN9CC with P22-like phages indicated that their lysogeny control regions and host cell lysis gene clusters show very low levels of identity, suggesting that lysogen formation and host cell lysis mechanisms may be diverse among phages in this group. Analysis of the expression of SPN9CC host cell lysis genes encoding holin, endolysin, and Rz/Rz1-like proteins individually or in combinations in S. Typhimurium and Escherichia coli hosts revealed that collaboration of these lysis proteins is important for the lysis of both hosts and that holin is a key protein. To further investigate the role of the lysogeny control region in phage SPN9CC, a ΔcI mutant (SPN9CCM) of phage SPN9CC was constructed. The mutant does not produce a cloudy center in the plaques, suggesting that this mutant phage is virulent and no longer temperate. Subsequent comparative one-step growth analysis and challenge assays revealed that SPN9CCM has shorter eclipse/latency periods and a larger burst size, as well as higher host cell lysis activity, than SPN9CC. The present work indicates the possibility of engineering temperate phages as promising biocontrol agents similar to virulent phages.
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