ArticleOsteoarthritis and cartilage2014
Identification of cartilage injury using quantitative multiphoton microscopy.
Article in Osteoarthritis and cartilage, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Spatial Distribution of Extracellular Vesicles, Autofluorescence and CD9 Positivity Around Chondrocytes in the Superficial Layer of Articular Cartilage.Journal of extracellular vesicles · 2025Article
- Combined flat-field and frequency filter approach to correcting artifacts of multichannel two-photon microscopy.Journal of biomedical optics · 2024Article
- Mask R-CNN provides efficient and accurate measurement of chondrocyte viability in the label-free assessment of articular cartilage.Osteoarthritis and cartilage open · 2023Article
- Articular Cartilage-From Basic Science Structural Imaging to Non-Invasive Clinical Quantitative Molecular Functional Information for AI Classification and Prediction.International journal of molecular sciences · 2023Review
- Nonlabeling and quantitative assessment of chondrocyte viability in articular cartilage with intrinsic nonlinear optical signatures.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Multiscale Strain as a Predictor of Impact-Induced Fissuring in Articular Cartilage.Journal of biomechanical engineering · 2017Article
- High-Resolution Methods for Diagnosing Cartilage Damage In Vivo.Cartilage · 2016Article
- Measuring microscale strain fields in articular cartilage during rapid impact reveals thresholds for chondrocyte death and a protective role for the superficial layer.Journal of biomechanics · 2015Article
- Joint-dependent response to impact and implications for post-traumatic osteoarthritis.Osteoarthritis and cartilage · 2015Article
- On fragmenting, densely mineralised acellular protrusions into articular cartilage and their possible role in osteoarthritis.Journal of anatomy · 2014Article
- Up-regulation of the chemo-attractive receptor ChemR23 and occurrence of apoptosis in human chondrocytes isolated from fractured calcaneal osteochondral fragments.Journal of anatomy · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveCartilage injury can lead to post-traumatic osteoarthritis (PTOA). Immediate post-trauma cellular and structural changes are not widely understood. Furthermore, current cellular-resolution cartilage imaging techniques require sectioning of cartilage and/or use of dyes not suitable for patient imaging. In this study, we used multiphoton microscopy (MPM) data with FDA-approved sodium fluorescein to identify and evaluate the pattern of chondrocyte death after traumatic injury.
methodMature equine distal metacarpal or metatarsal osteochondral blocks (OCBs) were injured by 30 MPa compressive loading delivered over 1 s. Injured and control sites were imaged unfixed and in situ 1 h post-injury with sodium fluorescein using rasterized z-scanning. MPM data was quantified in MATLAB, reconstructed in 3-D, and projected in 2-D to determine the damage pattern.
resultsMPM images (600 per sample) were reconstructed and analyzed for cell death. The overall distribution of cell death appeared to cluster into circular (n = 7) or elliptical (n = 4) patterns (p = 0.006). Dead cells were prevalent near cracks in the matrix, with only 26.3% (SE = 5.0%, p < 0.0001) of chondrocytes near cracks being viable.
conclusionThis study demonstrates the first application of MPM for evaluating cellular-scale cartilage injury in situ in live tissue, with clinical potential for detecting early cartilage damage. With this technique, we were able to uniquely observe two death patterns resulting from the same compressive loading, which may be related to local variability in matrix structure. These results also demonstrate proof-of-concept MPM diagnostic use in detecting subtle and early cartilage damage not detectable in any other way.
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