ArticleCellular signalling2014
The cyclic AMP phosphodiesterase RegA critically regulates encystation in social and pathogenic amoebas.
Article in Cellular signalling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Acanthamoeba Keratitis Management and Prognostic Factors: A Systematic Review.Journal of clinical medicine · 2025Review
- Encystment and Excystment Processes inPathogens (Basel, Switzerland) · 2025Review
- Emerging roles for diguanylate cyclase during the evolution of soma in dictyostelia.BMC ecology and evolution · 2023Article
- Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP.Biology open · 2023Article
- A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment.Nature communications · 2022Article
- Autophagy of the somatic stalk cells likely nurses the propagating spores of Dictyostelid social amoebas.Open research Europe · 2022Article
- Review
- From environmental sensing to developmental control: cognitive evolution in dictyostelid social amoebas.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Variables Affecting the Recovery ofPathogens (Basel, Switzerland) · 2021Article
- Discovery of Amoebicidal Compounds by Combining Computational and Experimental Approaches.Antimicrobial agents and chemotherapy · 2021Article
- Evolution of multicellularity in Dictyostelia.The International journal of developmental biology · 2019Review
- The transcription factor Spores Absent A is a PKA dependent inducer of Dictyostelium sporulation.Scientific reports · 2018Article
- Multiple Roots of Fruiting Body Formation in Amoebozoa.Genome biology and evolution · 2018Article
- Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia.EvoDevo · 2018Article
- The Origin of Animal Multicellularity and Cell Differentiation.Developmental cell · 2017Review
- Article
- Evolution of developmental signalling in Dictyostelid social amoebas.Current opinion in genetics & development · 2016Review
- Secreted Cyclic Di-GMP Induces Stalk Cell Differentiation in the Eukaryote Dictyostelium discoideum.Journal of bacteriology · 2016Review
- In Search of Enzymes with a Role in 3', 5'-Cyclic Guanosine Monophosphate Metabolism in Plants.Frontiers in plant science · 2016Review
- The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling.Genome biology and evolution · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Amoebas survive environmental stress by differentiating into encapsulated cysts. As cysts, pathogenic amoebas resist antibiotics, which particularly counteracts treatment of vision-destroying Acanthamoeba keratitis. Limited genetic tractability of amoeba pathogens has left their encystation mechanisms unexplored. The social amoeba Dictyostelium discoideum forms spores in multicellular fruiting bodies to survive starvation, while other dictyostelids, such as Polysphondylium pallidum can additionally encyst as single cells. Sporulation is induced by cAMP acting on PKA, with the cAMP phosphodiesterase RegA critically regulating cAMP levels. We show here that RegA is deeply conserved in social and pathogenic amoebas and that deletion of the RegA gene in P. pallidum causes precocious encystation and prevents cyst germination. We heterologously expressed and characterized Acanthamoeba RegA and performed a compound screen to identify RegA inhibitors. Two effective inhibitors increased cAMP levels and triggered Acanthamoeba encystation. Our results show that RegA critically regulates Amoebozoan encystation and that components of the cAMP signalling pathway could be effective targets for therapeutic intervention with encystation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.