Evidence map›Paper›PMID 24184017›Full record

ArticleJournal of microbiological methods2013

Comparative evaluation of vacuum-based surface sampling methods for collection of Bacillus spores.

M Worth Calfee, Laura J Rose, Stephen Morse, Dino Mattorano, Matt Clayton, Abderrahmane Touati, Nicole Griffin-Gatchalian, Christina Slone, Neal McSweeney

Open access · greenAbstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of microbiological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

M Worth CalfeeUS Environmental Protection Agency, National Homeland Security Research Center, Research Triangle Park, NC, USA. Electronic address: calfee.worth@epa.gov.
Laura J Rose
Stephen Morse
Dino Mattorano
Matt Clayton
Abderrahmane Touati
Nicole Griffin-Gatchalian
Christina Slone
Neal McSweeney
Arcadis (United States) · USCenters for Disease Control and Prevention · USEnvironmental Protection Agency · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

In this study, four commonly-used sampling devices (vacuum socks, 37 mm 0.8 μm mixed cellulose ester (MCE) filter cassettes, 37 mm 0.3 μm polytetrafluoroethylene (PTFE) filter cassettes, and 3M™ forensic filters) were comparatively evaluated for their ability to recover surface-associated spores. Aerosolized spores (~10(5)CFUcm(-2)) of a Bacillus anthracis surrogate were allowed to settle onto three material types (concrete, carpet, and upholstery). Ten replicate samples were collected using each vacuum method, from each material type. Stainless steel surfaces, inoculated simultaneously with test materials, were sampled with pre-moistened wipes. Wipe recoveries were utilized to normalize vacuum-based recoveries across trials. Recovery (CFUcm(-2)) and relative recovery (vacuum recovery/wipe recovery) were determined for each method and material type. Recoveries and relative recoveries ranged from 3.8 × 10(3) to 7.4 × 10(4)CFUcm(-2) and 0.035 to 1.242, respectively. ANOVA results indicated that the 37 mm MCE method exhibited higher relative recoveries than the other methods when used for sampling concrete or upholstery. While the vacuum sock resulted in the highest relative recoveries on carpet, no statistically significant difference was detected. The results of this study may be used to guide selection of sampling approaches following biological contamination incidents.

Indexed as

Environmental MicrobiologyVacuumBacillus anthracisBacteriological TechniquesColony Count, MicrobialSpecimen HandlingSpores, BacterialAnthraxBacillus anthracisBioterrorism agentSurface samplingVacuum sampling

Identifiers

PMID24184017
PMCPMC4780263
OpenAlexW2002449422

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.