ArticleThe Journal of cell biology1986
Organization of cytokeratin bundles by desmosomes in rat mammary cells.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
- The keratin-desmosome scaffold: pivotal role of desmosomes for keratin network morphogenesis.Cellular and molecular life sciences : CMLS · 2020Article
- A rim-and-spoke hypothesis to explain the biomechanical roles for cytoplasmic intermediate filament networks.Journal of cell science · 2017Review
- Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics.Cold Spring Harbor perspectives in biology · 2017Review
- Dissection of keratin network formation, turnover and reorganization in living murine embryos.Scientific reports · 2015Article
- Identification of novel principles of keratin filament network turnover in living cells.Molecular biology of the cell · 2004Article
- The apical submembrane cytoskeleton participates in the organization of the apical pole in epithelial cells.The Journal of cell biology · 1997Article
- Intermediate filaments as dynamic structures.Cancer metastasis reviews · 1996Review
- Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly.The Journal of cell biology · 1996Article
- Heteromeric connexons in lens gap junction channels.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Functional analysis of desmoplakin domains: specification of the interaction with keratin versus vimentin intermediate filament networks.The Journal of cell biology · 1993Article
- The morphological substrate of autonomic regulation of the bronchial epithelium.Virchows Archiv. A, Pathological anatomy and histopathology · 1993Article
- Identification of an epithelial protein related to the desmosome and intermediate filament network.The Journal of cell biology · 1992Article
- Keratin incorporation into intermediate filament networks is a rapid process.The Journal of cell biology · 1991Article
- Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains.The Journal of cell biology · 1991Article
- A protein antigenically related to nuclear lamin B mediates the association of intermediate filaments with desmosomes.The Journal of cell biology · 1990Article
- Terminally differentiating epithelial tissues in primary explant culture: a model of growth and development.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1990Review
- Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells.The Journal of cell biology · 1989Article
- Organization of desmosomal plaque proteins in cells growing at low calcium concentrations.The Journal of cell biology · 1988Article
- Kinetics of desmosome assembly in Madin-Darby canine kidney epithelial cells: temporal and spatial regulation of desmoplakin organization and stabilization upon cell-cell contact. II. Morphological analysis.The Journal of cell biology · 1988Article
- Structure and assembly of desmosome junctions: biosynthesis and turnover of the major desmosome components of Madin-Darby canine kidney cells in low calcium medium.The Journal of cell biology · 1987Article
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3 authors.
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Abstract
In a rat mammary epithelial cell line, LA-7, cytokeratin bundles recognized in immunofluorescence by a monoclonal antibody (24B42) disappear after trypsinization of cultures and are gradually reformed after replating. We have followed the time course of cytokeratin filament reappearance by growing cells in low calcium medium (0.1 mM) which prevents desmosome formation, and then shifting to high calcium (1.8 mM) to start the process. By fixing the cells at various intervals and staining them in immunofluorescence for 24B42 cytokeratin and for desmosomal proteins, we found that cell to cell contact and desmosome formation are prerequisites for keratin filament formation in these cells. EGTA treatment, by disassembling desmosomes, causes the cytokeratin filaments to disappear and the 24B42 protein to pass into a soluble form in this cell line, as ascertained by 100,000 g fractionation and immunoenzymatic assay. Cycloheximide treatment also causes cytokeratin filaments to disappear, indicating that protein synthesis is needed for normal filament maintenance. In another related cell line (106A-10a) and in HeLa cells, trypsinization and EGTA exposure do not cause a complete loss of 24B42 immunofluorescence, although distinct filaments disappear, indicating the presence in these cells of different organizing centers, besides desmosomes, for cytokeratin bundle formation. LA7 cells therefore seem to have a cytokeratin system strictly dependent on the presence of desmosomes, which act as an organizing center for filament assembly. 106A-10a cells (also rich in desmosomes) and HeLa cells (showing instead a reduced number of desmosomes) have a cytokeratin system partially or totally independent from that of desmosomes, with different organizing centers.
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