ArticlePlant cell reports1996
Regeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells.
Article in Plant cell reports, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Studies on growth dynamics of embryogenic cell suspension cultures of commercially important3 Biotech · 2018Article
- Regulation of expression of rice thaumatin-like protein: inducibility by elicitor requires promoter W-box elements.Plant cell reports · 2008Article
- Transformation of rice with long DNA-segments consisting of random genomic DNA or centromere-specific DNA.Transgenic research · 2007Article
- Arabitol dehydrogenase as a selectable marker for rice.Plant cell reports · 2005Article
- The classical Ubisch bodies carry a sporophytically produced structural protein (RAFTIN) that is essential for pollen development.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Expression of the REB transcriptional activator in rice grains improves the yield of recombinant proteins whose genes are controlled by a Reb-responsive promoter.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Transformation of rice mediated by Agrobacterium tumefaciens.Plant molecular biology · 1997Review
- Rice transformation: bombardment.Plant molecular biology · 1997Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regenerable embryogenic suspensions of elite Indica (group 1) rice varieties IR24, IR64, IR72 and an advanced Indica rice breeding line IR57311-95-2-3 were established within 6-8 weeks from 3-4 week old calli derived from mature seeds. Transgenic rice plants were obtained by introducing a plasmid carrying genes encoding hygromycin phosphotransferase (hph, conferring resistance to hygromycin B) and ß-glucuronidase (uidA), both driven by the CaMV 35S promoter, via particle bombardment of embryogenic suspensions. The effect of osmotic conditioning on transformation was evaluated. Regenerated plants were resistant to hygromycin B and expressed the uidA (GUS) gene. The growth of mother plants (R0) was normal and seeds were produced. Southern blot analysis of R0 and R1 plants showed that hygromycin resistant plants contained intact hph genes that were inherited in a Mendelian fashion. A protocol for a simple, efficient, repeatable, genotype- and environment-independent Indica rice transformation system is described.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.