Evidence map›Paper›PMID 24178350›Full record

ArticlePlant cell reports1996

Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.

E Sivamani, P Shen, N Opalka, R N Beachy, C M Fauquet

Abstract read
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In one paragraph

Article in Plant cell reports, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Transgene behaviour in populations of rice plants transformed using a new dual binary vector system: pGreen/pSoup.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003
    Article
  8. Article
  9. Rice transformation: bombardment.Plant molecular biology · 1997
    Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

E SivamaniDivision of Plant Biology, ILTAB/TSRI-ORSTOM, The Scripps Research Institute, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA.
P Shen
N Opalka
R N Beachy
C M Fauquet

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems, we have developed a procedure, using indica variety TN1 as a model that generates highly homogenous populations of embryogenic subcultured calli by selectively propagating a small number of regeneration-proficient calli derived from seeds. Thousands of embryogenic calli were produced from 50 seeds within 10 weeks. Ten to 20 independent R0 transgenic lines were regenerated per 500 embryogenic calli bombarded. The convenience and reliability offered by this transformation system has made transformation of indica rice a routine procedure.

Identifiers

PMID24178350

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.