Evidence map›Paper›PMID 24177325›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2014

MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.

Jie-Mei Wang, Jun Tao, Dan-Dan Chen, Jing-Jing Cai, Kaikobad Irani, Qinde Wang, Hong Yuan, Alex F Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed, 1 synthesis or guideline pooled it, 141 citations in OpenAlex.

  1. Pooled it
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  7. Review
  8. Role of exosome-derived miRNAs in diabetic wound angiogenesis.Molecular and cellular biochemistry · 2024
    Review
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  20. Gene therapy to enhance angiogenesis in chronic wounds.Molecular therapy. Nucleic acids · 2022
    Review

21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Jie-Mei WangFrom the Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA (J.-M.W., D.-D.C., Q.D.W., A.F.C.); Vascular Surgery Research, Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, PA (A.F.C.); Department of Hypertension and Vascular Disease, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China (J.-M.W., J.T.); Department of Cardiology and Center of Clinical Pharmacology, Third Xiangya Hospital, Central South University, Changsha, Hunan, China (J.-M.W., D.-D.C., J.-J.C., Q.D.W., H.Y., A.F.C.); and Division of Cardiovascular Medicine, Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA (K.I.).
Jun Tao
Dan-Dan Chen
Jing-Jing Cai
Kaikobad Irani
Qinde Wang
Hong Yuan
Alex F Chen
The First Affiliated Hospital, Sun Yat-sen University · CNVA Pittsburgh Healthcare System · USSun Yat-sen University · CN

Funding

Redox regulation of endothelial function and wound healingR01GM077352 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CHEN, ALEX F · 2006 to 2010
$1.3M
Function of ADAR1 in Hematopoietic and Leukemia Stem CellsR21CA158650 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WANG, QINGDE · 2012 to 2013
$352k
Tetrahydrobiopterin & eNOS uncoupling regulation of EPC function and wound healinI01RX000244 · VA · VETERANS HEALTH ADMINISTRATION · PI CHEN, ALEX F · 2012 to 2015
–
MicroRNA Regulation of Endothelial Progenitor Cell Function and Wound HealingI01RX000652 · VA · VETERANS HEALTH ADMINISTRATION · PI CHEN, ALEX F · 2015 to 2017
–
American Heart Association-American Stroke Association 0920110GAmerican Heart Association-American Stroke Association 13SDG16930098NCI NIH HHS 1R21CA158650NCI NIH HHS R21 CA158650NIGMS NIH HHS R01 GM077352RRD VA I01 RX000244RRD VA I01 RX000652
6 · The paper itself

Abstract

objectiveVascular precursor cells with angiogenic potentials are important for tissue repair, which is impaired in diabetes mellitus. MicroRNAs are recently discovered key regulators of gene expression, but their role in vascular precursor cell-mediated angiogenesis in diabetes mellitus is unknown. We tested the hypothesis that the microRNA miR-27b rescues impaired bone marrow-derived angiogenic cell (BMAC) function in vitro and in vivo in type 2 diabetic mice. APPROACH AND

resultsBMACs from adult male type 2 diabetic db/db and from normal littermate db/+ mice were used. miR-27b expression was decreased in db/db BMACs. miR-27b mimic improved db/db BMAC function, including proliferation, adhesion, tube formation, and delayed apoptosis, but it did not affect migration. Elevated thrombospondin-1 (TSP-1) protein in db/db BMACs was suppressed on miR-27b mimic transfection. Inhibition of miR-27b in db/+ BMACs reduced angiogenesis, which was reversed by TSP-1 small interfering RNA (siRNA). miR-27b suppressed the pro-oxidant protein p66(shc) and mitochondrial oxidative stress, contributing to its protection of BMAC function. miR-27b also suppressed semaphorin 6A to improve BMAC function in diabetes mellitus. Luciferase binding assay suggested that miR-27b directly targeted TSP-1, TSP-2, p66(shc), and semaphorin 6A. miR-27b improved topical cell therapy of diabetic BMACs on diabetic skin wound closure, with a concomitant augmentation of wound perfusion and capillary formation. Normal BMAC therapy with miR-27b inhibition demonstrated reduced efficacy in wound closure, perfusion, and capillary formation. Local miR-27b delivery partly improved wound healing in diabetic mice.

conclusionsmiR-27b rescues impaired BMAC angiogenesis via TSP-1 suppression, semaphorin 6A expression, and p66shc-dependent mitochondrial oxidative stress and improves BMAC therapy in wound healing in type 2 diabetic mice.

Indexed as

Neovascularization, PhysiologicWound HealingAnimalsBone Marrow CellsCase-Control StudiesCD36 AntigensCD47 AntigenCells, CulturedDiabetes Mellitus, Type 2Disease Models, AnimalFemaleHumansMaleMiceMice, Inbred C57BLMice, KnockoutCD36 AntigensCD47 AntigenCd47 protein, mouseMicroRNAsMIRN27 microRNA, humanMirn27 microRNA, mouseOligoribonucleotidesSema6a protein, mouseSemaphorinsShc1 protein, mouseShc Signaling Adaptor ProteinsSrc Homology 2 Domain-Containing, Transforming Protein 1Thbs1 protein, mouseThrombospondin 1Thrombospondin 2Thrombospondinsdiabetes mellitusmicroRNAstype 2wound healing

Identifiers

PMID24177325
PMCPMC5533613
OpenAlexW1966515321

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.