ArticleMicrobial cell factories2013
A low-cost, multiplexable, automated flow cytometry procedure for the characterization of microbial stress dynamics in bioreactors.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 59 citations in OpenAlex.
- A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors.NPJ systems biology and applications · 2026Article
- Comparative Approaches for Quantification of Product Yield in the Production of Recombinant Green Fluorescent Protein (GFPuv) Expressed inbioRxiv : the preprint server for biology · 2025Article
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- Programming living sensors for environment, health and biomanufacturing.Microbial biotechnology · 2021Article
- Following the community development of SIHUMIx - a new intestinalGut microbes · 2020Article
- Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.Microbial biotechnology · 2019Article
- Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity.Microbial cell factories · 2019Article
- In Silico Prediction of Large-Scale Microbial Production Performance: Constraints for Getting Proper Data-Driven Models.Computational and structural biotechnology journal · 2018Review
- Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond.Microbial biotechnology · 2017Article
- Evaluating Monitoring Strategies to Detect Precipitation-Induced Microbial Contamination Events in Karstic Springs Used for Drinking Water.Frontiers in microbiology · 2017Article
- Laboratory-Scale Simulation and Real-Time Tracking of a Microbial Contamination Event and Subsequent Shock-Chlorination in Drinking Water.Frontiers in microbiology · 2017Article
- Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth.Nature communications · 2016Article
- Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria.International journal of molecular sciences · 2016Review
- Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods and Applications.Frontiers in bioengineering and biotechnology · 2015Review
- The feasibility of automated online flow cytometry for in-situ monitoring of microbial dynamics in aquatic ecosystems.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobial cell population heterogeneity is now recognized as a major source of issues in the development and optimization of bioprocesses. Even if single cell technologies are available for the study of microbial population heterogeneity, only a few of these methods are available in order to study the dynamics of segregation directly in bioreactors. In this context, specific interfaces have been developed in order to connect a flow cytometer directly to a bioreactor for automated analyses. In this work, we propose a simplified version of such an interface and demonstrate its usefulness for multiplexed experiments.
resultsA low-cost automated flow cytometer has been used in order to monitor the synthesis of a destabilized Green Fluorescent Protein (GFP) under the regulation of the fis promoter and propidium iodide (PI) uptake. The results obtained showed that the dynamics of GFP synthesis are complex and can be attributed to a complex set of biological parameters, i.e. on the one hand the release of protein into the extracellular medium and its uptake modifying the activity of the fis promoter, and on the other hand the stability of the GFP molecule itself, which can be attributed to the protease content and energy status of the cells. In this respect, multiplexed experiments have shown a correlation between heat shock and ATP content and the stability of the reporter molecule.
conclusionThis work demonstrates that a simplified version of on-line FC can be used at the process level or in a multiplexed version to investigate the dynamics of complex physiological mechanisms. In this respect, the determination of new on-line parameters derived from automated FC is of primary importance in order to fully integrate the power of FC in dedicated feedback control loops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.